Recombinant CD34 antibody (AA 100-300)

Catalog No. ABIN7863990

The Mouse Monoclonal anti-CD34 antibody is suitable to detect CD34 in samples from Human. It has been validated for WB and IHC.

Quick Overview for Recombinant CD34 antibody (AA 100-300) (ABIN7863990)

Target: CD34

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD34 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: RHPCA1-8573

Product Details anti-CD34 Antibody

Binding Specificity: AA 100-300

Purpose: Recombinant CD34 (Hematopoietic Stem Cell & Endothelial Marker) Antibody

Specificity: This MAb recognizes a single chain, transmembrane, heavily glycosylated protein of 90-120kDa, which is identified as CD34. On the basis of differential sensitivity to degradation by specific enzymes, epitopes of monoclonal antibodies to CD34 are classified intoÇ 79oΩthree main categories, class I, class II and class III. It is a class II antibody whose epitope is resistant to neuraminidase but sensitive to glycoprotease and chymopapain.Ç 79oΩCD34 expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, CD34 is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. Its expression is also found in vascular endothelium. It appears that proliferating endothelial cells express this molecule more than the non-proliferating endothelial cells. Anti-CD34 labels > 85% of angiosarcoma and Kaposi s sarcoma, but with a lower specificity.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (around aa100-300) of human HPCA1 protein (exact sequence is proprietary)

Isotype: IgG1, lambda

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CD34

Target: CD34

Alternative Name: CD34

Background:

Hematopoietic Progenitor Cell Antigen, HPCA1, Mucosialin

Human tonsil or angiosarcoma. Heart.

Molecular Weight: 90-110kDa

Gene ID: 947, 374990

UniProt: P28906