Recombinant Topoisomerase II alpha antibody (AA 1300-1500)

Catalog No. ABIN7859104

The Mouse Monoclonal anti-Topoisomerase II alpha antibody (Clone rTOP2A-8339) (ABIN7859104) specifically detects Topoisomerase II alpha in WB and IHC. The antibody is reactive with Human samples.

Quick Overview for Recombinant Topoisomerase II alpha antibody (AA 1300-1500) (ABIN7859104)

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Topoisomerase II alpha antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: RTOP2A-8339

Product Details anti-Topoisomerase II alpha Antibody

Binding Specificity: AA 1300-1500

Purpose: Recombinant Topoisomerase II alpha (Proliferation & Drug-Resistance Marker) Antibody

Specificity: It recognizes a 170kDa protein, which is identified as topoisomerase II±. It shows no cross-reaction with Topoisomerase II ≤ or I. Topo II ± plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells. Topo II ± is also implicated in drug resistance of tumor cells and has been shown to be over-expressed in many human cancers. Decreased expression of Topo IIa is the predominant mechanism of resistance to several chemotherapeutic agents.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant human Topoisomerase II alpha fragment (aa1300-1500) (exact sequence is proprietary)

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Target Details for Topoisomerase II alpha

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Alternative Name: Topoisomerase II alpha

Background:

ATP hydrolyzing DNA topoisomerase II alfa, DNA gyrase, DNA topoisomerase (ATP hydrolyzing), DNA topoisomerase 2 alpha, DNA topoisomerase II 170kD, DNA topoisomerase II alpha, DNA Topoisomerase2, TOP2A, Topoisomerase DNA II alpha 170 kDa, TP2A

HeLa or Jurkat cells. Human lymph node or tonsil. A549.

Molecular Weight: 170kDa

Gene ID: 7153, 156346

UniProt: P11388

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases