Recombinant Topoisomerase II alpha antibody (AA 1352-1493)

Catalog No. ABIN7863463

The Rabbit Monoclonal anti-Topoisomerase II alpha antibody (Clone TOP2A-6570R) (ABIN7863463) specifically detects Topoisomerase II alpha in WB and IHC. The antibody is reactive with Human samples.

Quick Overview for Recombinant Topoisomerase II alpha antibody (AA 1352-1493) (ABIN7863463)

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Topoisomerase II alpha antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: TOP2A-6570R

Product Details anti-Topoisomerase II alpha Antibody

Binding Specificity: AA 1352-1493

Purpose: Recombinant Topoisomerase II alpha (Proliferation & Drug-Resistance Marker) Antibody

Specificity: The topoisomerase II-°™ isoform is a 170kDa nuclear protein and plays an important role in DNA synthesis and RNA transcription, as well as chromosomal segregation during mitosis. Topoisomerase II-°™ is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells, and has been shown to be over-expressed in many human cancers. Decreased expression of Topoisomerase II-°™ is the predominant mechanism of resistance to several chemotherapeutic agents.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant human Topoisomerase II alpha fragment (aa1352-1493) (exact sequence is proprietary)

Isotype: IgG, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Topoisomerase II alpha

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Alternative Name: Topoisomerase II alpha

Background:

ATP hydrolyzing DNA topoisomerase II alfa, DNA gyrase, DNA topoisomerase (ATP hydrolyzing), DNA topoisomerase 2 alpha, DNA topoisomerase II 170kD, DNA topoisomerase II alpha, Topoisomerase DNA II alpha 170 kDa, TP2A

HeLa or Jurkat cells. Human tonsil or esophagus.

Molecular Weight: 170kDa

Gene ID: 7153, 156346

UniProt: P11388

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases