Recombinant Topoisomerase II alpha antibody (AA 1431-1531)

Catalog No. ABIN7859125

The Rabbit Monoclonal anti-Topoisomerase II alpha antibody (Clone TOP2A-7148R) (ABIN7859125) specifically detects Topoisomerase II alpha in IHC. The antibody is reactive with Human samples.

Quick Overview for Recombinant Topoisomerase II alpha antibody (AA 1431-1531) (ABIN7859125)

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Topoisomerase II alpha antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: TOP2A-7148R

Product Details anti-Topoisomerase II alpha Antibody

Binding Specificity: AA 1431-1531

Purpose: Recombinant Topoisomerase II alpha (Proliferation & Drug-Resistance Marker) Antibody

Specificity: The topoisomerase II-± isoform is a 170kDa nuclear protein and plays an important role in DNA synthesis and RNA transcription, as well as chromosomal segregation during mitosis. Topoisomerase II-± is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells, and has been shown to be over-expressed in many human cancers. Decreased expression of Topoisomerase II-± is the predominant mechanism of resistance to several chemotherapeutic agents.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Synthetic peptide corresponding to Topo II α residues within aa1431-1531 of Topo II α

Isotype: IgG, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Target Details for Topoisomerase II alpha

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Alternative Name: Topoisomerase II alpha

Background:

ATP hydrolyzing DNA topoisomerase II alfa, DNA gyrase, DNA topoisomerase (ATP hydrolyzing), DNA topoisomerase 2 alpha, DNA topoisomerase II 170kD, DNA topoisomerase II alpha, Topoisomerase DNA II alpha 170 kDa, TP2A

Human tonsil or esophagus.

Molecular Weight: 170kDa

Gene ID: 7153, 156346

UniProt: P11388

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases