Recombinant Prostate Specific Antigen antibody (AA 150-250)

Catalog No. ABIN7862991

The Rabbit Monoclonal anti-Prostate Specific Antigen antibody is suitable to detect Prostate Specific Antigen in samples from Human. It has been validated for IHC.

Quick Overview for Recombinant Prostate Specific Antigen antibody (AA 150-250) (ABIN7862991)

Target: Prostate Specific Antigen (PSA)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Prostate Specific Antigen antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: KLK3-7128R

Product Details anti-Prostate Specific Antigen Antibody

Binding Specificity: AA 150-250

Purpose: Recombinant Prostate Specific Antigen (PSA) Antibody

Specificity: Prostate-specific antigen (PSA) is a single-chain glycoprotein of 237 amino acids containing approximately 8 % carbohydrate. It is a serine protease produced almost exclusively by prostatic epithelial cells.Immunohistochemically PSA is expressed in the highly specialized apically-superficial layer of female and male secretory cells of the prostate gland, and is readily demonstrated in adenocarcinomas of the prostate in about 99 % of the cases. There is a correlation between malignancy grade and intensity of staining, high grade carcinomas displaying weaker expression. About 1 % of poorly differentiated carcinomas have been negative for PSA.Due to the high specificity of PSA for prostatic glandular epithelium, it is very useful in identifying prostatic carcinoma in the prostate and in the adjacent organs often affected by epithelial malignancies, i.e. rectum and urinary bladder. PSA may be used in a panel together with NKX3.1 and Prostein, which are at least as sensitive and slightly more specific than PSA.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (around aa150-250) of human KLK3 protein (exact sequence is proprietary)

Isotype: IgG, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Prostate Specific Antigen

Target: Prostate Specific Antigen (PSA)

Alternative Name: Prostate Specific Antigen

Background:

Antigen, prostate-specific (APS), Gamma-seminoprotein, hK3, Kallikrein related peptidase 3, Kallikrein-3 (KLK3), KLK2A1, P-30 antigen, Semenogelase, Seminin

PC12 cells. Normal prostate or prostate carcinoma tissues.

Molecular Weight: 29kDa

Gene ID: 354, 171995

UniProt: P07288

Pathways: Complement System