MYCN antibody (AA 335-463)

Catalog No. ABIN7864358

This Mouse Monoclonal antibody specifically detects MYCN in ELISA and FACS. It exhibits reactivity toward Human.

Quick Overview for MYCN antibody (AA 335-463) (ABIN7864358)

Target: MYCN (N-Myc Proto-Oncogene Protein (MYCN))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MYCN antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS)

Clone: PCRP-MYCN-1A9

Product Details anti-MYCN Antibody

Binding Specificity: AA 335-463

Purpose: MYCN Antibody

Specificity: The v-Myc oncogene, initially identified in the MC29 avian retrovirus, causes myelocytomas, carcinomas, sarcomas and lymphomas, and belongs to a family of oncogenes conserved throughout evolution. In humans, the family consists of five genes: c-Myc, N-Myc, R-Myc, L-Myc and B-Myc. Amplification of the N-Myc gene has been found in human neuroblastomas and cell lines. Its amplification correlates well with the stage of neuroblastoma disease. Immunological studies have shown that the human N-Myc gene encodes a nuclear phosphoprotein that exhibits relatively short (30 min) half life in vivo. The prototype member of the family, c-Myc p67, binds DNA in a sequence-specific manner subsequent to dimerization with a second basic region helix-loop-helix leucine zipper motif protein, designated Max.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant protein domain (around aa335-463) of human MYCN protein

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Target Details for MYCN

Target: MYCN (N-Myc Proto-Oncogene Protein (MYCN))

Alternative Name: MYCN

Background:

Class E basic helix-loop-helix protein 37 (bHLHe37), MODED, N-myc proto-oncogene protein, Neuroblastoma derived v myc avian myelocytomatosis viral related oncogene, ODED, pp65/67, v myc (avian) myelocytomatosis viral related oncogene neuroblastoma derived

293T whole cell lysates or recombinant Human n-Myc protein.

Molecular Weight: 49.56 kDa

Gene ID: 4613, 25960

UniProt: P04198