ESR2 antibody (C-Term)

Catalog No. ABIN7862764

The Mouse Monoclonal anti-ESR2 antibody (Clone PPG5-25) (ABIN7862764) specifically detects ESR2 in WB, IHC and IF. The antibody is reactive with Human samples.

Quick Overview for ESR2 antibody (C-Term) (ABIN7862764)

Target: ESR2 (Estrogen Receptor 2 (ESR2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ESR2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: PPG5-25

Product Details anti-ESR2 Antibody

Binding Specificity: C-Term

Purpose: ER-beta (Estrogen Receptor ü5) Antibody

Specificity: This MAb specifically recognizes human estrogen receptor beta 5 (ERB5), a splice variant of the nuclear receptor hormone ER beta (ESR2), shown to exist in at least five isoforms (ERB1-ERB5). No cross-reactivity is seen with estrogen receptor beta 1 or 2 isoform. This gene encodes a member of the family of estrogen receptors and superfamily of nuclear receptor transcription factors. The gene product contains an N-terminal DNA binding domain and C-terminal ligand binding domain and is localized to the nucleus, cytoplasm, and mitochondria. Upon binding to 17beta-estradiol or related ligands, the encoded protein forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription. Some isoforms dominantly inhibit the activity of other estrogen receptor family members.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Tuberculin conjugated synthetic peptide (LLSHVRHARYAP), derived from the C-terminus of human Estrogen Receptor beta 5

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for ESR2

Target: ESR2 (Estrogen Receptor 2 (ESR2))

Alternative Name: ER-beta

Background:

Estrogen Receptor 2, Nuclear Receptor Subfamily 3 Group A Member 2 (NR3A2), Oestrogen Receptor Beta, Estrogen Receptor Beta, ER-BETA, ESTRB

colon or colon carcinoma., HCT cells. Normal liver

Molecular Weight: 59kDa

Gene ID: 2100, 660607

UniProt: Q92731

Pathways: Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling