Recombinant BRCA1 antibody (N-Term)

Catalog No. ABIN7865369

This Rabbit Monoclonal antibody specifically detects BRCA1 in IHC. It exhibits reactivity toward Human.

Quick Overview for Recombinant BRCA1 antibody (N-Term) (ABIN7865369)

Target: BRCA1 (Breast Cancer 1 (BRCA1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This BRCA1 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: BRCA1-13502R

Product Details anti-BRCA1 Antibody

Binding Specificity: N-Term

Purpose: Recombinant BRCA1 (Breast Marker) Antibody

Specificity: This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40 % of inherited breast cancers and more than 80 % of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment corresponding to the N-terminal region of human BRCA1 protein

Isotype: IgG, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for BRCA1

Target: BRCA1 (Breast Cancer 1 (BRCA1))

Alternative Name: BRCA1

Background:

BRCA1, Breast and ovarian cancer susceptibility protein 1, Breast Cancer 1 Early Onset, Breast cancer type 1 susceptibility protein, BROVCA1, IRIS, PNCA4, PPP1R53, Protein phosphatase 1 regulatory subunit 53, PSCP, RING finger protein 53, RNF53

MCF7 or A431 cells. Human breast, ovarian or renal cell carcinoma.

Molecular Weight: 220kDa

Gene ID: 672, 194143

UniProt: P38398

Pathways: Cell Division Cycle, DNA Damage Repair, Intracellular Steroid Hormone Receptor Signaling Pathway, Positive Regulation of Response to DNA Damage Stimulus