Recombinant Calnexin antibody (N-Term)

Catalog No. ABIN7860803

This Rabbit Monoclonal antibody specifically detects Calnexin in WB, IHC and ELISA. It exhibits reactivity toward Human.

Quick Overview for Recombinant Calnexin antibody (N-Term) (ABIN7860803)

Target: Calnexin (CANX)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Calnexin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Clone: CANX-9147R

Product Details anti-Calnexin Antibody

Binding Specificity: AA 1-300, N-Term

Purpose: Recombinant Calnexin (Endoplasmic Reticulum Marker) Antibody

Specificity: It recognizes a protein of 90 kDa, which is identified as Calnexin. Secretory and transmembrane proteins are synthesized on polysomes and translocate into the endoplasmic reticulum (ER) where they are often modified by the formation of disulfide bonds, amino-linked glycosylation and folding. To help proteins fold properly, the ER contains a pool of molecular chaperones including calnexin. It is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant N-terminal fragment of human Calnexin protein (around aa 1-300) (exact sequence is proprietary)

Isotype: IgG, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Target Details for Calnexin

Target: Calnexin (CANX)

Alternative Name: Calnexin

Background:

Calnexin, CANX, CNX, IP90, Major histocompatibility complex class I antigen-binding protein p88, P90

HeLa|MCF-7 or U2OS cells. Kidney or small intestine. Brain, Heart, Intestine, Kidney, Liver, Lung, Pancreas, Skin, Skeletal muscle and Stomach

Molecular Weight: 90kDa

Gene ID: 821, 567968

UniProt: P27824

Pathways: MAPK Signaling, Thyroid Hormone Synthesis