AMACR antibody

Catalog No. ABIN7862857

The Rabbit Monoclonal anti-AMACR antibody (Clone 13H4) (ABIN7862857) specifically detects AMACR in WB and IHC. The antibody is reactive with Human, Mouse and Guinea Pig samples.

Quick Overview for AMACR antibody (ABIN7862857)

Target: AMACR (alpha-Methylacyl-CoA Racemase (AMACR))

Reactivity: Human, Mouse, Guinea Pig

Host: Rabbit

Clonality: Monoclonal

Conjugate: This AMACR antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: 13H4

Product Details anti-AMACR Antibody

Purpose: AMACR / p504S (Prostate Cancer Marker) Antibody

Specificity: This antibody recognizes a protein of 54 kDa, which is identified as AMACR, also known as p504S. It is an enzyme that is involved in bile acid biosynthesis and æ-oxidation of branched-chain fatty acids. AMACR is essential in lipid metabolism. It is expressed in cells of premalignant high-grade prostatic intraepithelial neoplasia (HGPIN) and prostate adenocarcinoma. The majority of the carcinoma cells show a distinct granular cytoplasmic staining reaction. AMACR is present at low or undetectable levels in glandular epithelial cells of normal prostate and benign prostatic hyperplasia. A spotty granular cytoplasmic staining is seen in a few cells of the benign glands. AMACR is expressed in normal liver (hepatocytes), kidney (tubular epithelial cells) and gall bladder (epithelial cells). Expression has also been found in lung (bronchial epithelial cells) and colon (colonic surface epithelium). AMACR expression can also be found in hepatocellular carcinoma and kidney carcinoma. Past studies have also shown that AMACR is expressed in various colon carcinomas (well, moderately and poorly differentiated) and over expressed in prostate carcinoma.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human AMACR protein

Isotype: IgG, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody is stable for 24 months. ,Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for AMACR

Target: AMACR (alpha-Methylacyl-CoA Racemase (AMACR))

Alternative Name: AMACR

Background:

Alpha-methylacyl-CoA Racemase, CBAS4, Da1-8, Macr1, RACE, RM

HEK cells. Human kidney or prostate adenocarcinoma., HEK cells. Prostate Adenocarcinoma and human kidney or Mouse Kidney

Molecular Weight: 42kDa

Gene ID: 23600, 508343

UniProt: Q9UHK6

Pathways: Monocarboxylic Acid Catabolic Process