Recombinant Cyclin B1 antibody

Catalog No. ABIN7861160

This Rabbit Monoclonal antibody specifically detects Cyclin B1 in IHC. It exhibits reactivity toward Human.

Quick Overview for Recombinant Cyclin B1 antibody (ABIN7861160)

Target: Cyclin B1 (CCNB1)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Cyclin B1 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: CCNB1-9242R

Product Details anti-Cyclin B1 Antibody

Purpose: Recombinant Cyclin B1 (G2- & M-phase Cyclin) Antibody

Specificity: In eukaryotic cells, mitosis is initiated following the activation of a protein kinase known variously as maturation-promoting factor, M phase specific histone kinase or M-phase kinase. This protein kinase is composed of a catalytic subunit (Cdc2), a regulatory subunit (cyclin B) and a low molecular weight subunit (p13-Suc1). The Cdc/cyclin enzyme is subject to multiple levels of control, of which the regulation of the catalytic subunit by tyrosine phosphorylation is the best understood. Tyrosine phosphorylation inhibits the Cdc2/ cyclin B enzyme, and tyrosine dephos phorylation, occurring at the onset of mitosis, directly activates the pre-MPF complex. Evidence has established that B type cyclins not only act on M phase regulatory subunits of the Cdc2 protein kinase, but also activate the Cdc25A and Cdc25B endogenous tyrosine phosphatase, of which Cdc2 is the physio logical substrate. The specificity of this effect is shown by the inability of either cyclin A or cyclin D1 to display any such stimulation of Cdc25A or Cdc25B.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: His-tagged recombinant hamster CCNB1 protein

Isotype: IgG, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Target Details for Cyclin B1

Target: Cyclin B1 (CCNB1)

Alternative Name: Cyclin B1

Background:

CCNB, CCNB1, CCNB1_HUMAN, G2 Mitotic Specific Cyclin B1

Cell line in logarithmic growth phase. Human tonsil|Prostate or Ovarian Carcinoma.

Molecular Weight: 55-62kDa

Gene ID: 891, 23960

UniProt: P14635

Pathways: Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase