HSPD1 antibody

Catalog No. ABIN7862954

The Mouse Monoclonal anti-HSPD1 antibody (Clone LK2) (ABIN7862954) specifically detects HSPD1 in WB, IHC and IF. The antibody is reactive with Human, Mouse, Rat, Chicken, Monkey, Dog, Hamster, Cow, Rabbit, Pig, Sheep, Drosophila melanogaster and Xenopus laevis samples.

Quick Overview for HSPD1 antibody (ABIN7862954)

Target: HSPD1 (Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1))

Reactivity: Human, Mouse, Rat, Chicken, Monkey, Dog, Hamster, Cow, Rabbit, Pig, Sheep, Drosophila melanogaster, Xenopus laevis

Host: Mouse

Clonality: Monoclonal

Conjugate: This HSPD1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: LK2

Product Details anti-HSPD1 Antibody

Purpose: HSP60 (Heat Shock Protein 60) (Mitochondrial Marker) Antibody

Specificity: Recognizes a 60 kDa protein, identified as the heat shock protein 60 (hsp60). Its epitope is localized between aa 383-419 of human hsp60. A wide variety of environmental and pathophysiological stressful conditions trigger the synthesis of a family of proteins known as heat shock proteins (hsp s), more appropriately called as stress response proteins (srp s). hsp60 is a potential antigen in a number of autoimmune diseases. In human arthritis and in experimentally induced arthritis in animals, disease development coincides with the development of immune reactivity directed against not only bacterial hsp60, but also against its mammalian homolog.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant human HSP60 protein

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody is stable for 24 months. ,Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for HSPD1

Target: HSPD1 (Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1))

Alternative Name: HSP60

Background:

60 kDa heat shock protein mitochondrial, Chaperonin, 60-KD (CPN60), GROEL, HLD4, HSP65, HSPD1, HuCHA60, Mitochondrial matrix protein P1, P60 lymphocyte protein, Spastic paraplegia 13 (SPG13)

HeLa, Hep2 or HepG2 cells. Synovial biopsies from patients with juvenile chronic arthritis. Synovial lining layer is strongly positive for hsp60. Breast carcinoma.

Molecular Weight: 60kDa

Gene ID: 3329, 595053

UniProt: P10809

Pathways: Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity