IL-5 antibody

Catalog No. ABIN7863011

The Mouse Monoclonal anti-IL-5 antibody (Clone IL5-4161) (ABIN7863011) specifically detects IL-5 in IHC and FACS. The antibody is reactive with Human samples.

Quick Overview for IL-5 antibody (ABIN7863011)

Target: IL-5 (IL5) (Interleukin 5 (IL5))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL-5 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS)

Clone: IL5-4161

Product Details anti-IL-5 Antibody

Purpose: Interleukin-5 (IL-5) Antibody

Specificity: Interleukin-5, or IL-5, was originally discovered as a soluble T cell-derived factor, called T cell-replacing factor (TRF), that induced T cell-depleted activated B cells to secrete immunoglobulin. Native IL-5 is a disulfide-linked homodimer. IL-5 is initially synthesized as a precursor with a 19 amino acid signal peptide which is cleaved to form a 112 amino acid mature protein. Murine and human IL-5 exhibit 70 % sequence identity at the amino acid level. IL-5 exerts its biological activity through the IL-5 receptor (IL-5R), which is composed of at least two chains: an chain that binds IL-5 with low affinity and a chain that does not bind IL-5, but together with the IL-5 a chain, constitutes the high affinity IL-5 receptor. The chain is common to the IL-3, IL-5 and GM-CSF receptors and has been shown to signal through the JAK/Stat pathway.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment of human IL5 protein corresponding to the mature protein (exact sequence is proprietary)

Isotype: IgG2b, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for IL-5

Target: IL-5 (IL5) (Interleukin 5 (IL5))

Alternative Name: Interleukin-5

Background:

B-cell differentiation factor I, Colony stimulating factor, EDF, Eosinophil differentiation factor, Interleukin 5 (colony stimulating factor, eosinophil), T Cell Replacing Factor, TRF

Human thymus or tonsil tissue.

Molecular Weight: 17kDa

Gene ID: 3567, 2247

Pathways: JAK-STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour