MIF antibody

Catalog No. ABIN7864033

The Mouse Monoclonal anti-MIF antibody (Clone MIF-4285) (ABIN7864033) specifically detects MIF in WB. The antibody is reactive with Human samples.

Quick Overview for MIF antibody (ABIN7864033)

Target: MIF (Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MIF antibody is un-conjugated

Application: Western Blotting (WB)

Clone: MIF-4285

Product Details anti-MIF Antibody

Purpose: MIF (Macrophage Migration Inhibitory Factor) Antibody

Specificity: Macrophage migration inhibitory factor, known as MIF or glycosylationinhibiting factor, is a secreted, homotrimeric, pro-inflammatory cytokine that modulates macrophage and T cell function and is an important regulator of host response to infection. MIF is expressed at sites of inflammation, which suggests that it plays a role in regulating macrophage function in host defense. MIF is produced by the pituitary gland and is found in monocytes, macrophages, differentiating immunological cells in the eye lens and brain, and fibroblasts. Elevated levels of MIF protein are detected in the plasma of patients with severe sepsis or septic shock, a condition where MIF influences endotoxic shock by enhancing the production of other inflammatory cytokines including tumor necrosis factor é 79oΩ (TNFé 79oΩ), interleukin-1 (IL-1) and interferon-é 79oΩ (IFN-é 79oΩ). MIF promotes the systemic inflammatory response by counter-regulating glucocorticoid-mediated inhibition of immune-cell activation and proinflammatory cytokine production. MIF may mediate tissue destruction through the induction of proteinases.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment of human MIF protein (exact sequence is proprietary)

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for MIF

Target: MIF (Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF))

Alternative Name: MIF

Background:

GIF, GLIF, Glycosylation inhibiting factor, L-dopachrome isomerase, L-dopachrome tautomerase, Macrophage migration inhibitory factor (glycosylation-inhibiting factor), MIF, MIF protein, MMIF, Phenylpyruvate tautomerase

Human tonsil|thymus or prostate.

Molecular Weight: 13kDa

Gene ID: 4282, 407995

UniProt: P14174

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling