MBP antibody

Catalog No. ABIN7863117

This Rat Monoclonal antibody specifically detects MBP in WB, IHC, ELISA and IF. It exhibits reactivity toward Human, Cow, Horse and Chicken.

Quick Overview for MBP antibody (ABIN7863117)

Target: MBP (Myelin Basic Protein (MBP))

Reactivity: Human, Cow, Horse, Chicken

Host: Rat

Clonality: Monoclonal

Conjugate: This MBP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Clone: MBP-4274

Product Details anti-MBP Antibody

Purpose: Myelin Basic Protein Antibody

Specificity: Myelin basic protein (MBP) is the second most abundant protein in central nervous system (CNS) myelin: it comprises 30 % of the total protein and about 10 % of the dry weight of myelin. It is the only structural protein found so far to be essential for formation of CNS myelin, and has been called the executive molecule of myelin . MBP can interact with a number of polyanionic proteins including actin, tubulin, calmodulin, and clathrin, and negatively charged lipids, and acquires structure on binding to them. It may act as a membrane actin-binding protein, which might allow it to participate in transmission of extracellular signals to the cytoskeleton in oligodendrocytes and tight junctions in myelin. MBP may be applicable as a marker for oligodendrogliomas. MBP/4274 recognizes an epitope in the 36-50 region of MBP, useful in clinical diagnosis to detect MBP levels or myelination in human MBP.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Reacts with human MBP, recognizing epitopes in the 36-50 amino acid region (GILDSIGRFFGGDRG).

Isotype: IgG2b

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for MBP

Target: MBP (Myelin Basic Protein (MBP))

Alternative Name: Myelin Basic Protein

Background:

GDB, Golli MBP, myelin basic protein, Hemopoietic MBP, HMBPR, HUGO, MLD, Myelin A1 Protein, basic, Myelin Deficient, Myelin membrane encephalitogenic protein, SHI, Shiverer, SP

Human brain or astrocytoma tissue.

Molecular Weight: 13-21kDa

Gene ID: 4155, 551713

UniProt: P02686