Recombinant Osteopontin antibody

Catalog No. ABIN7864518

The Mouse Monoclonal anti-Osteopontin antibody is suitable to detect Osteopontin in samples from Human. It has been validated for WB and IHC.

Quick Overview for Recombinant Osteopontin antibody (ABIN7864518)

Target: Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Osteopontin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: ROSP-8856

Product Details anti-Osteopontin Antibody

Purpose: Recombinant Osteopontin (OSP) / Secreted Phosphoprotein 1 (SPP1) Antibody

Specificity: Osteopontin is a bone matrix protein and a cytokine secreted by activated T cells. Osteopontin (OPN, also designated bone sialoprotein 1, urinary stone protein, spp-1, é 79oΩ-1, nephropontin, uropontin) is an extracellular matrix cell adhesion phosphoglycoprotein. OPN is deposited into unmineralized matrix prior to calcification leading to localization at various tissue interfaces including cement lines, lamina limitans, and between collagen fibrils of fully matured hard tissues. While OPN is a major product of osteoblasts, it is also synthesized by brain and kidney cells. OPNs isolated from or secreted by various tissues have molecular weights due to post-translational modifications. OPN functions as a substrate for transglutaminase and is involved in cell adhesion, chemoattraction and immunomodulation.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length rat Spp1 protein

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Target Details for Osteopontin

Target: Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))

Alternative Name: Osteopontin

Background:

BNSP, Bone sialoprotein 1 (BSPI/BSP1), Early T lymphocyte activation 1 (ETA1), Nephropontin, immunoglobulin alpha 1 heavy chain constant region fusion protein, secreted phosphoprotein 1 (osteopontin bone sialoprotein I early T lymphocyte activation 1), Secreted phosphoprotein 1 (SPP1), CALPHA1 fusion, Urinary stone protein, Uropontin

Bone. Found in plasma. U87

Molecular Weight: 34.9kDa

Gene ID: 6696, 313

UniProt: P10451

Pathways: Regulation of Cell Size