Recombinant Vimentin antibody

Catalog No. ABIN7863497

This Mouse Monoclonal antibody specifically detects Vimentin in WB and IHC. It exhibits reactivity toward Human, Rat, Pig, Cow, Chicken, Dog, Cat and Horse.

Quick Overview for Recombinant Vimentin antibody (ABIN7863497)

Target: Vimentin (VIM)

Antibody Type: Recombinant Antibody

Reactivity: Human, Rat, Pig, Cow, Chicken, Dog, Cat, Horse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Vimentin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: RVIM-6575

Product Details anti-Vimentin Antibody

Purpose: Recombinant Vimentin (Mesenchymal Cell Marker) Antibody

Specificity: This MAb reacts with a 58 kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP s) such as desmin, keratin, neurofilament, and glial fibrillary acid protein.Anti-vimentin alone is of limited value as a diagnostic tool, however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human Vimentin protein

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Vimentin

Target: Vimentin (VIM)

Alternative Name: Vimentin

Background:

VIM

Jurkat or HeLa cells. Human lymph node or tonsil., Raji, U-87

Molecular Weight: 57-60kDa

Gene ID: 7431, 455493

UniProt: P08670

Pathways: Caspase Cascade in Apoptosis