Recombinant MUC1 antibody

Catalog No. ABIN7863166

The Mouse Monoclonal anti-MUC1 antibody is suitable to detect MUC1 in samples from Human. It has been validated for IHC and WB.

Quick Overview for Recombinant MUC1 antibody (ABIN7863166)

Target: MUC1 (Mucin 1 (MUC1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MUC1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Clone: RMUC1-4418

Product Details anti-MUC1 Antibody

Purpose: Recombinant MUC1 / CA15-3 / EMA / CD227 (Epithelial Marker) Antibody

Specificity: In Western blotting, it recognizes proteins in MW range of 265-400 kDa, identified as different glycoforms of EMA. EMA may provide a protective layer on epithelial cells against bacterial and enzyme attack. In immunohistochemical assays, it superbly stains routine formalin/paraffin carcinomas. Anti-EMA antibody is a useful marker for staining many carcinomas. It stains normal and neoplastic cells from various tissues, including mammary epithelium, sweat glands and colorectal carcinoma. Hepatocellular carcinoma, adrenal carcinoma and embryonal carcinomas are consistently EMA negative, so keratin positivity with negative EMA favors one of these tumors. EMA is frequently positive in meningioma, which can be useful when distinguishing it from other intracranial neoplasms such as schwannomas. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.

Purification: 200ug/ml of Ab purified from by Protein A.

Immunogen: Recombinant full-length human MUC1 protein

Isotype: IgG2a, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for MUC1

Target: MUC1 (Mucin 1 (MUC1))

Alternative Name: MUC1

Background:

Breast carcinoma-associated antigen DF3, CA15-3, Carcinoma-associated mucin Episialin, Epithelial Membrane Antigen, H23AG, KL-6, MAM6, MUC-1, MUC-1/SEC, MUC-1/X, MUC1-alpha, MUC1-beta, MUC1-CT, MUC1-NT, MUC1/ZD, Mucin 1 cell surface associated, Mucin-1 subunit beta, Peanut-reactive urinary mucin, PEM, PEMT, Polymorphic epithelial mucin, PUM, Tumor-associated epithelial membrane antigen, Tumor-associated mucin

Colon, MCF-7 or MDA-231 cells. Breast, ovarian or endometrial carcinoma. Kidney, Lung.

Molecular Weight: 265-400kDa

Gene ID: 4582, 89603

UniProt: P15941

Pathways: Negative Regulation of intrinsic apoptotic Signaling