TIA1 antibody (AA 279-380)

Catalog No. ABIN7863451

The Mouse Monoclonal anti-TIA1 antibody is suitable to detect TIA1 in samples from Human. It has been validated for WB, IF and FACS.

Quick Overview for TIA1 antibody (AA 279-380) (ABIN7863451)

Target: TIA1 (TIA1 Cytotoxic Granule-Associated RNA Binding Protein (TIA1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TIA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: TIA1-1313

Product Details anti-TIA1 Antibody

Binding Specificity: AA 279-380

Purpose: TIA1 (T-Cell-Restricted Intracellular Antigen-1) Antibody

Specificity: TIA-1 (T-cell intracytoplasmic antigen) is a cytoplasmic granule-associated protein, expressed in lymphocytes processing cytolytic potential. TIA-1 is a member of an RNA-binding protein family and possesses nucleolytic activity against cytotoxic lymphocyte (CTL) target cells. It has been suggested that this protein may be involved in the induction of apoptosis as it preferentially recognizes poly(A) homopolymers and induces DNA fragmentation in CTL targets. The major granule-associated species is a 15 kDa protein thought to be derived from the carboxyl terminus of the 40 kDa product by proteolytic processing. TIA1 antibody labels cytotoxic T cells and natural killer cells (NK cells). It is also expressed in T-cell lymphoma, large granular lymphocyte (LGL) leukemia and hairy cell leukemia. TIA1 expression in T-cell malignancies may help in differentiating LGL leukemia (high expression) from T-cell lymphocytosis and other T-cell diseases (low expression). TIA1 may also be used to label tumor-infiltrating lymphocytes in the study of immune response to malignancies.

Purification: 200ug/ml of Ab Purified from rabbit anti-serum by Protein A.

Immunogen: Recombinant human TIA1 fragment of 102 amino acid residues (aa279-380)

Isotype: IgG2b, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for TIA1

Target: TIA1 (TIA1 Cytotoxic Granule-Associated RNA Binding Protein (TIA1))

Alternative Name: TIA1

Background:

Cytotoxic granule associated RNA binding protein 1, mTIA-1, Nucleolysin TIA1 isoform p40, p40-TIA-1, RNA binding protein TIA1, T-cell-restricted intracellular antigen-1 (TIA1), TIA-1, TIA1, TIA1 cytotoxic granule associated RNA binding protein, TIAL1, TIAR, WDM

HePG2, PC3 or HeLa cells. Human tonsil.

Molecular Weight: 40kDa

Gene ID: 7072, 413123

UniProt: P31483