Recombinant CD34 antibody (AA 285-385)

Catalog No. ABIN7864211

The Rabbit Monoclonal anti-CD34 antibody (Clone HPCA1-4393R) (ABIN7864211) specifically detects CD34 in IHC. The antibody is reactive with Human samples.

Quick Overview for Recombinant CD34 antibody (AA 285-385) (ABIN7864211)

Target: CD34

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CD34 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: HPCA1-4393R

Product Details anti-CD34 Antibody

Binding Specificity: AA 285-385

Purpose: Recombinant CD34 (Hematopoietic Stem Cell & Endothelial Marker) Antibody

Specificity: CD34 (also named myeloid progenitor cell antigen) is a heavily glycosylated type I transmembrane protein. There are two forms of the CD34 protein, resulting from alternative splicing. The functions of CD34 is largely unknown, but recent evidence suggests a role for CD34 in cell adhesion and inhibition of hematopoiesis. It is suggested that CD34 is a signaling molecule involved in maintenance of a phenotypically plastic state in undifferentiated cells. CD34 is found in most endothelia, expressed on the luminal surface and membrane processes interdigitating between endothelial cells, but is absent from large veins and arteries. CD34 is furthermore expressed in fibroblast-like dendritic cells in, e.g., portal tracts of the liver, Peyer's patches, and in healing wounds. In smooth muscle cells, a variable CD34 staining is found. In tumor tissues, CD34 is detected in myeloid blasts in myelodysplastic syndrome and acute myeloid leukemia in most cases as well as lymphoblasts in most cases of B-acute lymphoblastic leukemia. Mature B- and T-cell lymphomas and leukemias are CD34 negative. The majority of vascular tumors, including hemangiosarcoma and Kaposi sarcoma are CD34positive. In a panel, CD34 staining is useful for the classification of myeloid and lymphoid neoplasms as well as spindle cell neoplasms (particularly identification of gastrointestinal stromal tumor and hemangiosarcoma).

Purification: 200ug/ml of recombinant MAb purified by Protein A/G.

Immunogen: Synthetic peptide corresponding to CD34 residues within aa285-385 of CD34 was used as an immunogen

Isotype: IgG, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CD34

Target: CD34

Alternative Name: CD34

Background:

Hematopoietic Progenitor Cell Antigen, HPCA1, Mucosialin

Jurkat or KG-1 cells. Human tonsil or angiosarcoma.

Molecular Weight: 41kDa

Gene ID: 947, 374990

UniProt: P28906