MUC1 antibody

Catalog No. ABIN7863167

This Mouse Monoclonal antibody specifically detects MUC1 in IHC, WB, FACS and IF. It exhibits reactivity toward Human and Mouse.

Quick Overview for MUC1 antibody (ABIN7863167)

Target: MUC1 (Mucin 1 (MUC1))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This MUC1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: Mc5

Product Details anti-MUC1 Antibody

Purpose: MUC1 / CA15-3 / EMA / CD227 (Epithelial Marker) Antibody

Specificity: The mucin antigen recognized by Mc-5 is a glycosylated molecule with a molecular mass of 400 kD. The sequence to which this antibody binds is Thr-Arg-Pro-Ala-Pro. Although EMA is primarily located in mammary gland epithelium, other normal epithelia (e.g., lung) will also react against EMA antibody. Staining, however, is the strongest in mammary epithelia. The combination of positive staining for keratin with negative EMA can be used to phenotype the above-mentioned epithelial tumors. Recognizes the mucin antigen that is present in most epithelial tissues (breast, colonic, bronchial, gastric, etc.) This antibody does not recognize myelopoietic, lymphopoietic, fibroblasts or sarcomatous cells.

Purification: 200ug/ml of recombinant MAb purified by Protein A/G.

Immunogen: Delipidated human milk fat globules

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for MUC1

Target: MUC1 (Mucin 1 (MUC1))

Alternative Name: MUC1

Background:

Breast carcinoma-associated antigen DF3, CA15-3, Carcinoma-associated mucin Episialin, H23AG, KL-6, MAM6, MUC-1, MUC-1/SEC, MUC-1/X, MUC1-alpha, MUC1-beta, MUC1-CT, MUC1-NT, MUC1/ZD, Mucin-1 subunit beta, Peanut-reactive urinary mucin, PEM, PEMT, Polymorphic epithelial mucin, PUM, Tumor-associated epithelial membrane antigen

Human kidney, lung, breast carcinoma or normal breast.

Molecular Weight: 400kDa

Gene ID: 4582, 89603

UniProt: P15941

Pathways: Negative Regulation of intrinsic apoptotic Signaling