Recombinant Cytokeratin 7 antibody (AA 1-100)

Catalog No. ABIN7865508

The Rabbit Monoclonal anti-Cytokeratin 7 antibody is suitable to detect Cytokeratin 7 in samples from Human. It has been validated for WB and IHC.

Quick Overview for Recombinant Cytokeratin 7 antibody (AA 1-100) (ABIN7865508)

Target: Cytokeratin 7 (KRT7) (Keratin 7 (KRT7))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Cytokeratin 7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: KRT7-13672R

Product Details anti-Cytokeratin 7 Antibody

Binding Specificity: AA 1-100

Expression System: HEK-293 Cells

Purpose: Recombinant Cytokeratin 7 (Glandular and Transitional Epithelial Marker) Antibody

Specificity: Cytokeratin 7 in humans is a protein encoded by the KRT7 gene. It is always as a keratin pair with Cytokeratin 19. Cytokeratin 7 is expressed on most ductal and glandular epithelium including lung, breast, bladder and female genital tract, but not in most gastrointestinal epithelium, prostate, hepatocyte and squamous epithelium. In tumor tissues, its expression is absent in colon cancer, prostate cancer and squamous carcinomas. Cytokeratin 7 is often used in concert with Cytokeratin 20 and CDX-2 to aid in distinguishing ovarian, pulmonary and breast carcinomas (CK7+) from colon carcinomas (CK7-).

Purification: Ab produced in HEK293 cell mammalian-based expression system.

Immunogen: Recombinant fragment (around aa1-100) of human KRT7 protein (exact sequence is proprietary)

Isotype: IgG, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Cytokeratin 7

Target: Cytokeratin 7 (KRT7) (Keratin 7 (KRT7))

Alternative Name: Cytokeratin 7

Background:

CK-7, K2C7, Keratin 55K Type II Cytoskeletal, Keratin Simple Epithelial Type 1 K7, Keratin Type II Cytoskeletal 7, Krt2-7, KRT7, Sarcolectin, SCL, Type II Mesothelial Keratin K7, Type-II Keratin Kb7

Pancreas: CK7 staining is at least weak to moderate cytoplasmic intercalating ducts and strong in large pancreatic ducts, SK-BR-3. HeLa.

Molecular Weight: 55kDa

Gene ID: 3855, 411501

UniProt: P08729