PKA antibody (pSer338)

Catalog No. ABIN7862487

The Rabbit Polyclonal anti-PKA antibody is suitable to detect PKA in samples from Human, Mouse and Rat. It has been validated for ELISA, IF and IHC.

Quick Overview for PKA antibody (pSer338) (ABIN7862487)

Target: PKA (Protein Kinase A (PKA))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKA antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC)

Product Details anti-PKA Antibody

Binding Specificity: pSer338

Purpose: Rabbit Anti-PKAα cat (phospho Ser338) Polyclonal Antibody

Specificity: Phospho-PKAα cat (S338) Polyclonal Antibody detects endogenous levels of PKAα cat protein only when phosphorylated at S338.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human PKAalpha cat Phospho-Ser338

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC 1:100-1:300,ELISA 1:5000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for PKA

Target: PKA (Protein Kinase A (PKA))

Alternative Name: PKAα

Background: PRKACA, PKACA, cAMP-dependent protein kinase catalytic subunit alpha, PKA C-alphaPRKACA (protein kinase cAMP-activated catalytic subunit alpha) encodes one of the catalytic subunits of protein kinase A, which exists as a tetrameric holoenzyme with two regulatory subunits and two catalytic subunits, in its inactive form. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. cAMP-dependent phosphorylation of proteins by protein kinase A is important to many cellular processes, including differentiation, proliferation, and apoptosis. Constitutive activation of this gene caused either by somatic mutations, or genomic duplications of regions that include this gene, have been associated with hyperplasias and adenomas of the adrenal cortex and are linked to corticotropin-independent Cushing's syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. Tissue-specific isoforms that differ at the N-terminus have been described, and these isoforms may differ in the post-translational modifications that occur at the N-terminus of some isoforms.

Gene ID: 5566

UniProt: P17612

Pathways: SARS-CoV-2 Protein Interactome