CD22 antibody

Catalog No. ABIN7865582

This Mouse Monoclonal antibody specifically detects CD22 in FACS, IHC, IF and ICC. It exhibits reactivity toward Human.

Quick Overview for CD22 antibody (ABIN7865582)

Target: CD22 (CD22 Molecule (CD22))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD22 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: MYG13

Product Details anti-CD22 Antibody

Purpose: anti-CD22 mouse monoclonal, MYG13, purified

Purification: affinity chromatography

Immunogen: RAJI cells

Isotype: IgG1 kappa

Application Details

Application Notes:

ICC/IF_dilution: 1:100-1:200 (0.5-1.0 μg/mL)

FACS_dilution: 0.5-1.0 μg/million cells in 0.1 ml

IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: store at 2 - 8 °C

Target Details for CD22

Target: CD22 (CD22 Molecule (CD22))

Alternative Name: CD22

Background: MYG13 reacts with high affinity to CD22, which is expressed in the cytoplasma of all B-cells, appearing as early as cell-surface CD19 during B-cell development. It's present on the surface of most mature sIg+ B-cells with especially high expression on hairy cell and prolymphocytic leukemia cells. CD22 is a member of the immunoglobulin super-family and acts as an adhesion molecule: BL-CAM. On frozen sections, CD22 is found highly expressed in follicular mantle and marginal zone B-cells, while germinal centre B-cell relatively weakly. Positive control: Raji, Daudi, IM9, JY25 and human peripheral blood lymphocytes or tonsil.