CD55 antibody

Catalog No. ABIN7865618

This Mouse Monoclonal antibody specifically detects CD55 in WB, FACS, IHC, IF and ICC. It exhibits reactivity toward Human and Pig.

Quick Overview for CD55 antibody (ABIN7865618)

Target: CD55 (Complement Decay-Accelerating Factor (CD55))

Reactivity: Human, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD55 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 143-30

Product Details anti-CD55 Antibody

Purpose: anti-CD55 mouse monoclonal, 143-30, purified

Purification: affinity chromatography

Immunogen: stimulated human leucocytes

Isotype: IgG1 kappa

Application Details

Application Notes:

ICC/IF_dilution: 1:100-1:200 (0.5-1.0 μg/mL)

FACS_dilution: 0.5-1.0 μg/million cells in 0.1 ml

WB_dilution: 1:50-1:100 (1-2 μg/mL)

IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: store at 2 - 8 °C

Target Details for CD55

Target: CD55 (Complement Decay-Accelerating Factor (CD55))

Alternative Name: CD55

Background: 143-30 Reacts with CD55 or DAF (Decay Accelerating Factor). All leucocytes as well as human erythrocytes, fibroblasts, platelets, endothelial cells and neuroectodermal cells are positive for DAF, a glycosylphosphatidylinositol anchored (GPI-anchored) member of the membrane bound complement regulatory proteins that inhibit autologous complement cascade activation. CD55 also serves as receptor for CD97 and for echovirus and coxsackie B virus. DAF is deficient in both granulocytes and monocytes in patients with paroxysmal nocturnal haemoglobinuria. 143-30 Has been clustered in Vienna at the IVth International Workshop on human leucocyte differentiation antigens. Positive control: Jurkat, HUT-78, K562, YT, U937, MG63, and human lymphocytes, human lymph nodes and tonsils.

Pathways: Complement System, Regulation of Leukocyte Mediated Immunity