FYCO1 antibody (AA 1-1019)

Catalog No. ABIN7867920

The Rabbit Polyclonal anti-FYCO1 antibody is suitable to detect FYCO1 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IHC and FACS.

Quick Overview for FYCO1 antibody (AA 1-1019) (ABIN7867920)

Target: FYCO1 (FYVE and Coiled-Coil Domain Containing 1 (FYCO1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FYCO1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-FYCO1 Antibody

Binding Specificity: AA 1-1019

Purpose: FYCO1 Antibody / FYVE and coiled-coil domain containing 1

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human FYCO1 recombinant protein (Position: M1-K1019) was used as the immunogen for the FYCO1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the FYCO1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the FYCO1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for FYCO1

Target: FYCO1 (FYVE and Coiled-Coil Domain Containing 1 (FYCO1))

Alternative Name: FYCO1

Background: FYCO1 antibody detects FYVE and coiled-coil domain containing 1, encoded by the FYCO1 gene. FYVE and coiled-coil domain containing 1 is a cytoplasmic protein that functions as an autophagy adaptor, linking autophagosomes to microtubule-based transport systems. FYCO1 antibody provides researchers with a key reagent for studying autophagy, vesicle trafficking, and disease mechanisms involving impaired cellular clearance.

FYVE and coiled-coil domain containing 1 contains a FYVE domain that binds phosphatidylinositol-3-phosphate on autophagosome membranes and a LC3-interacting region that recruits autophagy machinery. Research using FYCO1 antibody has shown that the protein couples autophagosomes to kinesin motors, promoting their transport along microtubules toward the cell periphery. This process facilitates autophagosome maturation and fusion with lysosomes.

Studies with FYCO1 antibody have revealed that loss-of-function mutations in FYCO1 cause autosomal recessive congenital cataracts. In lens epithelial cells, defective autophagy due to impaired FYCO1 activity leads to accumulation of damaged proteins and organelles, resulting in lens opacity. This genetic association highlights the clinical relevance of FYCO1 in ocular development and disease.

Dysregulation of FYVE and coiled-coil domain containing 1 has also been implicated in neurodegeneration and infection. Research using FYCO1 antibody has shown that altered expression affects autophagosome trafficking in neurons, contributing to accumulation of aggregates in neurodegenerative conditions. Pathogens such as Mycobacterium tuberculosis can manipulate FYCO1-mediated pathways, underscoring its significance in host-pathogen interactions.

FYCO1 antibody is widely applied in immunofluorescence, immunohistochemistry, and western blotting. Immunofluorescence highlights co-localization with LC3-positive autophagosomes, immunohistochemistry demonstrates tissue-specific expression, and western blotting quantifies levels in cells and tissues. These applications make FYCO1 antibody indispensable in autophagy and vesicle transport research.

By providing validated FYCO1 antibody reagents, NSJ Bioreagents supports studies into autophagy, cellular clearance, and disease. Detection of FYVE and coiled-coil domain containing 1 provides researchers with insight into how adaptor proteins regulate vesicle trafficking and pathology.

UniProt: Q9BQS8

Pathways: SARS-CoV-2 Protein Interactome