PYCARD antibody (AA 1-195)

Catalog No. ABIN7868349

The Rabbit Polyclonal anti-PYCARD antibody is suitable to detect PYCARD in samples from Human. It has been validated for WB, ELISA, IF, FACS and IHC (p).

Quick Overview for PYCARD antibody (AA 1-195) (ABIN7868349)

Target: PYCARD (PYD and CARD Domain Containing (PYCARD))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PYCARD antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PYCARD Antibody

Binding Specificity: AA 1-195

Purpose: TMS1 Antibody / Target of methylation-induced silencing 1 / PYCARD

Purification: Antigen affinity purified

Immunogen: E. coli-derived recombinant human protein (amino acids M1-S195) was used as the immunogen for the TMS1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the TMS1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the TMS1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for PYCARD

Target: PYCARD (PYD and CARD Domain Containing (PYCARD))

Alternative Name: TMS1

Background: PYCARD, often referred to as ASC (Apoptosis-associated speck-like protein containing a CARD) and Target of methylation-induced silencing 1 (TMS1), is a protein that in humans is encoded by the PYCARD gene. This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm, however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene.

UniProt: Q9ULZ3

Pathways: Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation, Inflammasome