ASCL1 antibody (AA 1-196)

Catalog No. ABIN7868352

The Rabbit Polyclonal anti-ASCL1 antibody is suitable to detect ASCL1 in samples from Zebrafish (Danio rerio). It has been validated for WB.

Quick Overview for ASCL1 antibody (AA 1-196) (ABIN7868352)

Target: ASCL1 (Achaete-Scute Complex Homolog 1 (Drosophila) (ASCL1))

Reactivity: Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ASCL1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ASCL1 Antibody

Binding Specificity: AA 1-196

Purpose: Zebrafish Ascl1a Antibody / Achaete-scute homolog 1

Purification: Antigen affinity chromatography

Immunogen: E. coli-derived zebrafish Ascl1a recombinant protein (amino acids M1-F196) was used as the immunogen for the Zebrafish Ascl1a antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Optimal dilution of the Zebrafish Ascl1a antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the Zebrafish Ascl1a antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for ASCL1

Target: ASCL1 (Achaete-Scute Complex Homolog 1 (Drosophila) (ASCL1))

Alternative Name: Ascl1a

Background: Achaete-scute family bHLH transcription factor 1a (Ascl1a) is a basic helix-loop-helix (bHLH) transcription factor that plays a crucial role in neurogenesis and cell fate determination. In zebrafish (Danio rerio), Ascl1a is a key regulator of neural progenitor proliferation and differentiation, particularly within the central nervous system, retina, and intestinal epithelium.

Ascl1a acts downstream of proneural signaling pathways and promotes the commitment of progenitor cells to a neuronal lineage. It is expressed in neural precursor cells during embryonic development and is reactivated in regenerative contexts. In the zebrafish retina and brain, Ascl1a is essential for the regeneration of neurons following injury, making it a prominent factor in regenerative neurobiology.

Zebrafish Ascl1a is widely used as a model for studying the molecular mechanisms of neuronal differentiation, stem cell biology, and tissue regeneration. Its expression is tightly regulated by signaling pathways such as Notch, and its function is conserved across vertebrates, including in mammalian neurogenesis.

UniProt: Q90259

Pathways: Dopaminergic Neurogenesis