SRA1 antibody (AA 1-224)

Catalog No. ABIN7868857

The Rabbit Polyclonal anti-SRA1 antibody (ABIN7868857) specifically detects SRA1 in WB, ELISA, IF, FACS and ICC. The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for SRA1 antibody (AA 1-224) (ABIN7868857)

Target: SRA1 (Steroid Receptor RNA Activator 1 (SRA1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SRA1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-SRA1 Antibody

Binding Specificity: AA 1-224

Purpose: SRA1 Antibody / Steroid receptor RNA activator 1

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human SRA1 recombinant protein (Position: M1-S224) was used as the immunogen for the SRA1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the SRA1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the SRA1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for SRA1

Target: SRA1 (Steroid Receptor RNA Activator 1 (SRA1))

Alternative Name: SRA1

Background: SRA1 antibody detects Steroid receptor RNA activator 1, a multifunctional molecule that exists as both a non-coding RNA and a protein coactivator involved in nuclear receptor signaling. The UniProt recommended name is Steroid receptor RNA activator 1 (SRA1). This gene produces both an RNA transcript functioning as a transcriptional coactivator and a protein (SRAP) that modulates gene expression by interacting with hormone receptor complexes.

Functionally, SRA1 antibody identifies a 236-amino-acid nuclear protein that binds steroid hormone receptors such as estrogen receptor (ESR1), androgen receptor (AR), and glucocorticoid receptor (NR3C1). SRA1 enhances receptor-dependent transcription by stabilizing coactivator complexes containing p160 family members (NCOA1-3) and histone acetyltransferases such as CBP/p300. The RNA form of SRA1 acts as a scaffold that promotes chromatin remodeling and transcriptional activation.

The SRA1 gene is located on chromosome 5q31.3 and is broadly expressed in hormone-responsive tissues including breast, uterus, prostate, and skeletal muscle. Its expression is induced by steroid hormones, forming a positive feedback loop that amplifies nuclear receptor signaling. SRA1 plays key roles in reproductive physiology, energy metabolism, and cellular differentiation.

Pathologically, aberrant SRA1 expression contributes to hormone-dependent cancers and metabolic syndromes. Overexpression enhances estrogen and androgen signaling, driving tumor growth in breast and prostate cancer. Mutations or reduced expression can impair receptor-mediated gene regulation and contribute to infertility or metabolic dysfunction. Research using SRA1 antibody supports studies in transcriptional regulation, nuclear receptor biology, and hormone signaling.

SRA1 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect transcriptional coactivators. NSJ Bioreagents provides SRA1 antibody reagents optimized for use in endocrinology, gene expression, and cancer biology research.

Structurally, Steroid receptor RNA activator 1 protein (SRAP) contains RNA recognition and protein interaction domains that allow dual regulatory capacity through RNA-protein networks. Its modular architecture supports cooperative interactions with multiple transcriptional cofactors. This antibody enables detailed analysis of SRA1's function in steroid hormone receptor signaling and coactivation mechanisms.

UniProt: Q9HD15

Pathways: EGFR Signaling Pathway, Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development