EGLN1 antibody (AA 1-24)

Catalog No. ABIN7868900

This Mouse Monoclonal antibody specifically detects EGLN1 in WB and IHC (p). It exhibits reactivity toward Human.

Quick Overview for EGLN1 antibody (AA 1-24) (ABIN7868900)

Target: EGLN1 (Egl-9 Family Hypoxia Inducible Factor 1 (EGLN1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This EGLN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 366G-76-3

Product Details anti-EGLN1 Antibody

Binding Specificity: AA 1-24

Purpose: EGLN1 Antibody / PHD2

Purification: Protein G affinity chromatography

Immunogen: Amino acids 1-24 of human PHD2/EGLN1 were used as the immunogen for the EGLN1 antibody.

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal dilution of the EGLN1 / PHD2 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the EGLN1 / PHD2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for EGLN1

Target: EGLN1 (Egl-9 Family Hypoxia Inducible Factor 1 (EGLN1))

Alternative Name: EGLN1

Background: 366G/76/3 recognises human prolyl hydroxylase 2 (PHD2), a 46 kDa enzyme expressed abundantly in all tissues with the highest expression in testis. Hypoxia inducible factor-1 (HIF-1) is a transcriptional complex, consisting of an alpha and beta subunit, which plays a key role in coordinating the cellular response to hypoxia. During normal oxygen conditions, the alpha subunit of HIF-1 is rapidly degraded, however when hypoxia occurs this degradation is suppressed and HIF-1 activates the transcription of various genes important for survival and adaptation to hypoxia. Prolyl hydroxylase 2 catalyses the hydroxylation of specific prolyl residues within the HIF-1 alpha subunit, thereby targeting this subunit for degradation.

UniProt: Q9GZT9

Pathways: cAMP Metabolic Process, Warburg Effect