Nkx2-2 antibody (AA 1-269)

Catalog No. ABIN7869010

The Rabbit Polyclonal anti-Nkx2-2 antibody (ABIN7869010) specifically detects Nkx2-2 in WB. The antibody is reactive with Zebrafish (Danio rerio) samples.

Quick Overview for Nkx2-2 antibody (AA 1-269) (ABIN7869010)

Target: Nkx2-2 (NK2 Homeobox 2 (Nkx2-2))

Reactivity: Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nkx2-2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Nkx2-2 Antibody

Binding Specificity: AA 1-269

Purpose: Zebrafish Nkx2.2a Antibody / Nkx2.2

Purification: Antigen affinity chromatography

Immunogen: E. coli-derived zebrafish Nkx2.2a recombinant protein (amino acids M1-W269) was used as the immunogen for the Zebrafish Nkx2.2a antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Optimal dilution of the Zebrafish Nkx2.2a antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the Zebrafish Nkx2.2a antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for Nkx2-2

Target: Nkx2-2 (NK2 Homeobox 2 (Nkx2-2))

Alternative Name: Nkx2.2a

Background: Nkx2.2a is a homeobox transcription factor that plays a critical role in the development of the central nervous system and pancreas in zebrafish. It belongs to the NK class of homeodomain proteins and functions by regulating gene expression involved in cell fate determination and patterning during embryogenesis.

Zebrafish Nkx2.2a is one of two paralogs, the other being Nkx2.2b, resulting from a genome duplication event in teleosts. Nkx2.2a is considered a functional ortholog of the human NKX2 dash 2 gene. Both proteins are highly conserved and share similar developmental roles, particularly in the specification of ventral neural progenitors and in endocrine pancreas formation.

In zebrafish embryos, Nkx2.2a is expressed in the ventral neural tube, including the regions that give rise to motor neurons, oligodendrocyte precursors, and serotonergic neurons. It acts downstream of sonic hedgehog signaling and is essential for defining the identity of specific neuronal subtypes. Nkx2.2a also contributes to the early development of the pancreas, where it helps regulate differentiation of insulin producing beta cells and other endocrine cell types.

Loss of Nkx2.2a function in zebrafish leads to defects in neuronal patterning and impaired endocrine pancreas development, mirroring the phenotypes observed in mammalian systems. Due to its conservation with the human NKX2 dash 2 gene and its involvement in key developmental processes, zebrafish Nkx2.2a is widely used as a model to study neurogenesis, pancreas biology, and transcriptional regulation in vertebrates.

UniProt: Q90481

Pathways: Dopaminergic Neurogenesis