TIGAR antibody (AA 1-269)

Catalog No. ABIN7869011

This Rabbit Polyclonal antibody specifically detects TIGAR in WB, ELISA, IHC and IF. It exhibits reactivity toward Mouse and Rat.

Quick Overview for TIGAR antibody (AA 1-269) (ABIN7869011)

Target: TIGAR (TP53 induced glycolysis regulatory phosphatase (TIGAR))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TIGAR antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-TIGAR Antibody

Binding Specificity: AA 1-269

Purpose: TIGAR Antibody / TP53-induced glycolysis and apoptosis regulator

Purification: Immunogen affinity purified

Immunogen: E.coli-derived mouse TIGAR recombinant protein (Position: M1-H269) was used as the immunogen for the TIGAR antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the TIGAR antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the TIGAR antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for TIGAR

Target: TIGAR (TP53 induced glycolysis regulatory phosphatase (TIGAR))

Alternative Name: TIGAR

Background: TIGAR antibody detects TP53-induced glycolysis and apoptosis regulator, a fructose-2,6-bisphosphatase-like enzyme that links p53 signaling to metabolic control and cell survival. TIGAR modulates glycolytic flux and antioxidant defense, protecting cells from oxidative stress. The TIGAR antibody is widely used in cancer metabolism, apoptosis, and redox biology research to study metabolic adaptation, p53 regulation, and energy homeostasis.

TIGAR is encoded by the TIGAR gene located on human chromosome 12p13.31. The protein is approximately 270 amino acids in length and structurally resembles the bisphosphatase domain of phosphofructokinase-2/fructose-2,6-bisphosphatase (PFKFB) enzymes. However, TIGAR lacks kinase activity and instead functions primarily as a fructose-2,6-bisphosphatase, reducing levels of fructose-2,6-bisphosphate and thereby suppressing glycolysis while promoting the pentose phosphate pathway (PPP).

The TIGAR antibody detects a 30 kilodalton band by western blot and shows cytoplasmic and nuclear localization under immunofluorescence. Through its enzymatic activity, TIGAR decreases glycolytic intermediates and redirects glucose metabolism toward the PPP, enhancing production of NADPH and glutathione for antioxidant defense. This metabolic shift protects cells against oxidative stress and apoptosis, particularly under conditions of DNA damage or metabolic stress.

Regulated directly by p53, TIGAR expression serves as a metabolic checkpoint balancing energy generation and redox homeostasis. In cancer, overexpression of TIGAR supports tumor cell survival by reducing reactive oxygen species (ROS) and maintaining mitochondrial integrity. Conversely, TIGAR inhibition sensitizes cells to oxidative damage and enhances the efficacy of chemotherapeutic agents.

Beyond cancer metabolism, TIGAR plays roles in neuroprotection, cardiac remodeling, and ischemic tolerance. In neurons, TIGAR preserves mitochondrial function and reduces cell death after hypoxic injury. In cardiac tissue, TIGAR activation supports energy recovery following ischemia. These observations establish TIGAR as a critical regulator of metabolic plasticity and stress resilience.

NSJ Bioreagents provides a validated TIGAR antibody optimized for western blot, immunohistochemistry, and cell metabolism studies, supporting research into p53-dependent metabolic reprogramming, oxidative stress control, and therapeutic metabolism targeting.

UniProt: Q8BZA9

Pathways: Warburg Effect