TIGAR antibody (AA 1-270)

Catalog No. ABIN7869019

The Rabbit Polyclonal anti-TIGAR antibody (ABIN7869019) specifically detects TIGAR in WB, ELISA, IHC, IF, ICC and FACS. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for TIGAR antibody (AA 1-270) (ABIN7869019)

Target: TIGAR (TP53 induced glycolysis regulatory phosphatase (TIGAR))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TIGAR antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-TIGAR Antibody

Binding Specificity: AA 1-270

Purpose: TIGAR Antibody / TP53-induced glycolysis and apoptosis regulator

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human TIGAR recombinant protein (Position: M1-R270) was used as the immunogen for the TIGAR antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the TIGAR antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the TIGAR antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for TIGAR

Target: TIGAR (TP53 induced glycolysis regulatory phosphatase (TIGAR))

Alternative Name: TIGAR

Background: TIGAR antibody detects TP53-induced glycolysis and apoptosis regulator, a key metabolic enzyme that modulates glycolysis, antioxidant defense, and cell survival. Encoded by the TIGAR gene on chromosome 12p13.3, this protein was identified as a transcriptional target of the tumor suppressor p53. TIGAR functions as a fructose-2,6-bisphosphatase, reducing levels of fructose-2,6-bisphosphate in the cytoplasm and thereby inhibiting glycolysis while promoting the pentose phosphate pathway. This metabolic shift enhances NADPH production, which supports antioxidant defense and protects cells against reactive oxygen species (ROS).

TIGAR contains a bisphosphatase domain homologous to that of the PFKFB family of enzymes but functions independently to regulate metabolic flux. By lowering intracellular fructose-2,6-bisphosphate, it decreases glycolytic activity and reduces lactate production while increasing glucose utilization for nucleotide synthesis and redox balance. This activity allows TIGAR to protect cells from oxidative stress and apoptosis under p53-mediated stress responses. It also contributes to autophagy regulation by balancing energy production and redox homeostasis.

The TIGAR antibody is used in metabolism, cancer, and redox biology research to investigate metabolic reprogramming and p53-dependent signaling. Western blot analysis typically identifies a 30 kilodalton band corresponding to TIGAR, while immunofluorescence shows cytoplasmic and perinuclear staining in metabolically active cells. Because TIGAR regulates the balance between glycolysis and antioxidant pathways, this antibody is a valuable tool for studying stress responses, metabolic adaptation, and tumor metabolism.

In cancer, TIGAR overexpression can support tumor cell survival by reducing oxidative stress, contributing to chemoresistance and metabolic flexibility. Conversely, TIGAR deficiency enhances ROS accumulation and apoptosis, sensitizing cells to stress. NSJ Bioreagents provides the TIGAR antibody validated for its applications, ensuring consistent detection in studies of metabolism, apoptosis, and cancer biology.

UniProt: Q9NQ88

Pathways: Warburg Effect