GATM antibody (AA 1-279)

Catalog No. ABIN7869040

The Rabbit Polyclonal anti-GATM antibody (ABIN7869040) specifically detects GATM in WB, IHC, ELISA, IF and FACS. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for GATM antibody (AA 1-279) (ABIN7869040)

Target: GATM (Glycine Amidinotransferase (L-Arginine:glycine Amidinotransferase) (GATM))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GATM antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-GATM Antibody

Binding Specificity: AA 1-279

Purpose: GATM Antibody / Glycine amidinotransferase mitochondrial

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human GATM recombinant protein (Position: M1-E279) was used as the immunogen for the GATM antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the GATM antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the GATM antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for GATM

Target: GATM (Glycine Amidinotransferase (L-Arginine:glycine Amidinotransferase) (GATM))

Alternative Name: GATM

Background: GATM antibody detects Glycine amidinotransferase, mitochondrial, an enzyme that catalyzes the first committed step in creatine biosynthesis. The UniProt recommended name is Glycine amidinotransferase, mitochondrial (GATM), also known as L-arginine:glycine amidinotransferase or AGAT. GATM transfers an amidino group from arginine to glycine, producing guanidinoacetate, which is subsequently methylated to creatine by GAMT. Creatine and phosphocreatine serve as rapid energy buffers in tissues with fluctuating ATP demand, including muscle and brain.

Functionally, GATM antibody identifies a 423-amino-acid mitochondrial matrix enzyme that catalyzes a reversible transamidination reaction dependent on pyridoxal phosphate (PLP) as a cofactor. This reaction provides the foundation for creatine synthesis, linking amino acid metabolism with cellular energy storage. The enzyme's activity supports energy homeostasis by ensuring adequate creatine production for high-energy phosphate transfer through the phosphocreatine system.

The GATM gene is located on chromosome 15q21.1 and encodes a homotetrameric enzyme targeted to mitochondria via an N-terminal transit peptide. GATM is primarily expressed in kidney, pancreas, and liver, where it supplies guanidinoacetate for systemic creatine synthesis. In muscle and neural tissues, creatine generated downstream of GATM activity is phosphorylated by creatine kinase to form phosphocreatine, maintaining ATP levels during high metabolic activity. Deficiency or mutation of GATM causes creatine deficiency syndrome, characterized by neurological impairment and muscle weakness.

GATM antibody is widely used in studies of amino acid metabolism, energy homeostasis, and mitochondrial function. It is a valuable marker for assessing creatine biosynthesis and mitochondrial enzyme integrity. Reduced GATM activity is linked to metabolic and neurological disorders, while upregulation has been observed under energy stress and oxidative conditions. The enzyme's function connects arginine metabolism, urea cycle intermediates, and ATP regeneration.

Structurally, GATM consists of an active-site lysine residue forming a Schiff base with PLP, enabling catalysis of amidino transfer. The enzyme forms stable homotetramers that ensure efficient substrate channeling and regulation. GATM activity is modulated by substrate availability and feedback inhibition from creatine levels. It interacts with mitochondrial transport proteins to coordinate substrate exchange across the inner membrane. A GATM antibody supports research applications including western blotting, immunohistochemistry, and metabolic flux analysis, providing insights into energy metabolism and mitochondrial health.

NSJ Bioreagents provides GATM antibody reagents validated for use in energy metabolism, mitochondrial biology, and enzymology research.

UniProt: P50440