PPP1R12A antibody (AA 1-40)

Catalog No. ABIN7869302

This Rabbit Polyclonal antibody specifically detects PPP1R12A in WB and IHC (p). It exhibits reactivity toward Zebrafish (Danio rerio).

Quick Overview for PPP1R12A antibody (AA 1-40) (ABIN7869302)

Target: PPP1R12A (Myosin Phosphatase, Target Subunit 1 (PPP1R12A))

Reactivity: Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPP1R12A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PPP1R12A Antibody

Binding Specificity: AA 1-40

Purpose: Zebrafish Ppp1r12a Antibody / Mypt1

Purification: Antigen affinity chromatography

Immunogen: E. coli-derived zebrafish Ppp1r12a recombinant protein (amino acids M1-D40) was used as the immunogen for the Zebrafish Ppp1r12a antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Optimal dilution of the Zebrafish Ppp1r12a antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the Zebrafish Ppp1r12a antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for PPP1R12A

Target: PPP1R12A (Myosin Phosphatase, Target Subunit 1 (PPP1R12A))

Alternative Name: Ppp1r12a

Background: Ppp1r12a, or protein phosphatase one regulatory subunit twelve a, is a regulatory protein that controls the activity of protein phosphatase one, a major serine threonine phosphatase involved in numerous cellular processes. In zebrafish, Ppp1r12a plays a critical role in regulating cytoskeletal organization, cell contractility, and morphogenesis during development.

Zebrafish Ppp1r12a is the ortholog of the human PPP1R12A gene, which encodes the protein also known as myosin phosphatase target subunit one. This protein directs protein phosphatase one to myosin light chains and other cytoskeletal targets, enabling dynamic control of actomyosin contraction and cell movement. It is involved in signaling pathways that govern smooth muscle relaxation, cell shape changes, and cell migration.

During zebrafish development, Ppp1r12a is expressed in tissues undergoing active morphogenetic movements and structural rearrangement, such as the developing somites, neural tube, and heart. It contributes to proper tissue organization by regulating the phosphorylation state of cytoskeletal components and by modulating mechanical tension within cells.

Disruption of Ppp1r12a function in zebrafish has been shown to affect cell adhesion, motility, and organ formation, highlighting its importance in developmental processes. Due to its conservation across vertebrates and its central role in regulating phosphatase activity, zebrafish Ppp1r12a is a useful model for studying signal transduction, cytoskeletal regulation, and developmental disorders involving cell morphology and contractility.

UniProt: Q6DRG7

Pathways: M Phase