TOX3 antibody (AA 1-560)

Catalog No. ABIN7869475

The Rabbit Polyclonal anti-TOX3 antibody (ABIN7869475) specifically detects TOX3 in WB, FACS, IF, ELISA and ICC. The antibody is reactive with Human samples.

Quick Overview for TOX3 antibody (AA 1-560) (ABIN7869475)

Target: TOX3 (TOX High Mobility Group Box Family Member 3 (TOX3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TOX3 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), ELISA, Immunocytochemistry (ICC)

Product Details anti-TOX3 Antibody

Binding Specificity: AA 1-560

Purpose: TOX3 Antibody / TOX high mobility group box family member 3

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human TOX3 recombinant protein (Position: M1-Q560) was used as the immunogen for the TOX3 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the TOX3 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the TOX3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for TOX3

Target: TOX3 (TOX High Mobility Group Box Family Member 3 (TOX3))

Alternative Name: TOX3

Background: TOX3 antibody detects TOX high mobility group box family member 3, a nuclear transcriptional cofactor involved in calcium-dependent gene regulation, chromatin remodeling, and neuronal survival. The UniProt recommended name is TOX high mobility group box family member 3 (TOX3). This protein functions as a chromatin-modifying transcriptional regulator and is expressed primarily in brain tissue, where it contributes to synaptic maintenance and neuroprotection.

Functionally, TOX3 antibody identifies a 576-amino-acid nuclear protein containing a high mobility group (HMG) box domain that binds to DNA and alters chromatin structure. TOX3 acts as a transcriptional co-regulator by interacting with CREB-binding protein (CBP), CITED1, and other transcriptional activators, enhancing the expression of calcium-dependent genes. It modulates neuronal plasticity and calcium signaling pathways crucial for learning, memory, and neuroprotection.

The TOX3 gene is located on chromosome 16q12.1 and encodes a transcription factor expressed in central nervous system neurons, endocrine tissues, and certain epithelial cells. Through chromatin remodeling, TOX3 facilitates access of transcriptional machinery to DNA and influences the balance between gene activation and repression. Its HMG box domain induces DNA bending, promoting nucleosome repositioning and chromatin flexibility.

TOX3 has attracted attention as a genetic susceptibility locus in breast cancer, where specific polymorphisms near the TOX3 gene are associated with increased risk. In tumors, aberrant TOX3 expression can alter transcriptional programs governing cell differentiation and apoptosis. In the nervous system, TOX3 helps protect neurons from excitotoxicity by modulating calcium-regulated gene networks. Reduced expression has been linked to increased vulnerability to neurodegenerative injury and impaired neuronal survival.

TOX3 antibody is widely used in neuroscience, epigenetics, and cancer biology research. It is suitable for western blotting, immunohistochemistry, and chromatin immunoprecipitation to detect TOX3 expression and DNA binding activity. This antibody supports studies of transcriptional regulation, calcium signaling, and chromatin dynamics. In oncology, it aids in exploring the functional relevance of TOX3 variants in hormone-responsive cancers.

Structurally, TOX3 contains an HMG DNA-binding domain and transcriptional regulatory regions that interact with coactivators and histone acetyltransferases. Its activity is modulated by calcium-dependent phosphorylation and nuclear localization signals. NSJ Bioreagents provides TOX3 antibody reagents validated for use in chromatin regulation, neurobiology, and transcriptional control research.

UniProt: O15405

Pathways: Chromatin Binding