PCYOX1 antibody (AA 101-452)

Catalog No. ABIN7869899

This Rabbit Polyclonal antibody specifically detects PCYOX1 in WB, IHC, ELISA and FACS. It exhibits reactivity toward Human.

Quick Overview for PCYOX1 antibody (AA 101-452) (ABIN7869899)

Target: PCYOX1 (Prenylcysteine Oxidase 1 (PCYOX1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PCYOX1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Product Details anti-PCYOX1 Antibody

Binding Specificity: AA 101-452

Purpose: PCYOX1 Antibody / Prenylcysteine oxidase 1

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human PCYOX1 recombinant protein (Position: R101-D452) was used as the immunogen for the PCYOX1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the PCYOX1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the PCYOX1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for PCYOX1

Target: PCYOX1 (Prenylcysteine Oxidase 1 (PCYOX1))

Alternative Name: PCYOX1

Background: PCYOX1 antibody detects Prenylcysteine oxidase 1, an enzyme that catalyzes the final step in the degradation of prenylated proteins. Encoded by the PCYOX1 gene on chromosome 2p21, this flavin-dependent oxidase converts prenylcysteine residues-generated from protein turnover-into cysteine sulfinic acid, carbon dioxide, and prenyl aldehydes. PCYOX1 thereby contributes to the detoxification and recycling of lipid-modified proteins and regulates cellular redox balance.

Structurally, PCYOX1 is a 501-amino-acid enzyme of approximately 57 kilodaltons containing a flavin adenine dinucleotide (FAD)-binding domain responsible for oxidative catalysis. It localizes to the endoplasmic reticulum and cytosol, where it associates with membranes to metabolize prenylated cysteine derivatives derived from small GTPases such as Ras and Rho. This activity maintains proper turnover of prenylated signaling proteins and limits accumulation of reactive intermediates that could disrupt cell membranes.

The PCYOX1 antibody is widely used in metabolism, enzymology, and redox biology research to study lipid modification turnover, oxidative regulation, and prenylated protein catabolism. Western blot analysis detects a 57 kilodalton band corresponding to PCYOX1, while immunofluorescence shows cytosolic and reticular staining in metabolically active tissues such as liver and kidney. This antibody provides a reliable tool for assessing enzyme function in lipid processing and oxidative homeostasis.

Functionally, PCYOX1 acts downstream of protein prenylation pathways that modify small GTPases for membrane targeting and signal transduction. By degrading prenylcysteine products, it prevents the accumulation of toxic intermediates and contributes to lipid-derived aldehyde metabolism. PCYOX1 activity is linked to oxidative stress response, with increased expression observed in conditions of lipid peroxidation and metabolic overload. Dysregulation of PCYOX1 expression has been associated with atherosclerosis and metabolic disease, implicating its role in vascular oxidative stress. The PCYOX1 antibody provides a valuable reagent for studying the interplay between protein modification, lipid metabolism, and oxidative signaling. NSJ Bioreagents validates this antibody for its applications, ensuring accurate and reproducible performance for metabolic and enzymatic research.

UniProt: Q9UHG3