LEPREL1 antibody (AA 106-494)

Catalog No. ABIN7869995

This Rabbit Polyclonal antibody specifically detects LEPREL1 in WB, ELISA, IHC, FACS, ICC and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for LEPREL1 antibody (AA 106-494) (ABIN7869995)

Target: LEPREL1 (Leprecan Like Protein 1 (LEPREL1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LEPREL1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-LEPREL1 Antibody

Binding Specificity: AA 106-494

Purpose: P3H2 Antibody / Prolyl 3-hydroxylase 2

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human P3H2 recombinant protein (Position: E106-R494) was used as the immunogen for the P3H2 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the P3H2 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the P3H2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for LEPREL1

Target: LEPREL1 (Leprecan Like Protein 1 (LEPREL1))

Alternative Name: P3H2

Background: P3H2 antibody detects Prolyl 3-hydroxylase 2, an endoplasmic reticulum enzyme responsible for hydroxylating proline residues in collagen and collagen-like proteins. Encoded by the P3H2 gene on chromosome 3q28, this enzyme belongs to the 2-oxoglutarate-dependent dioxygenase family and functions in post-translational modification of extracellular matrix proteins. P3H2 catalyzes the formation of 3-hydroxyproline at specific sites in fibrillar collagens, contributing to the stability, folding, and assembly of collagen triple helices.

P3H2 localizes to the lumen of the endoplasmic reticulum, where it operates as part of a multi-enzyme complex with cartilage-associated protein (CRTAP) and cyclophilin B (PPIB). This complex ensures proper hydroxylation and folding of procollagen molecules prior to secretion. The enzymatic reaction requires Fe2+, 2-oxoglutarate, and ascorbate as cofactors. Loss of P3H2 activity leads to underhydroxylated collagen and defects in connective tissue organization.

The P3H2 antibody is used in extracellular matrix, connective tissue, and skeletal biology research to study collagen modification and protein maturation. Western blot analysis detects a 90 kilodalton band corresponding to P3H2, while immunofluorescence reveals reticular ER localization consistent with its role in collagen biosynthesis. This antibody allows characterization of collagen-modifying enzymes and post-translational hydroxylation mechanisms.

Altered expression or mutation of P3H2 has been implicated in connective tissue disorders, osteogenesis imperfecta, and fibrosis, where abnormal collagen hydroxylation compromises structural integrity. The P3H2 antibody is an essential reagent for exploring collagen maturation, matrix assembly, and disease-associated remodeling. NSJ Bioreagents offers this antibody validated for western blotting, immunofluorescence, and immunohistochemistry, ensuring accuracy in studies of extracellular matrix biology and protein modification.

UniProt: Q8IVL5