PDK1 antibody (AA 114-436)

Catalog No. ABIN7870193

This Rabbit Polyclonal antibody specifically detects PDK1 in WB, FACS and ELISA. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for PDK1 antibody (AA 114-436) (ABIN7870193)

Target: PDK1 (Pyruvate Dehydrogenase Kinase 1 (PDK1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDK1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA

Product Details anti-PDK1 Antibody

Binding Specificity: AA 114-436

Purpose: Pyruvate dehydrogenase kinase 1 Antibody / PDK1

Purification: Antigen affinity purified

Immunogen: An E.coli-derived human recombinant protein (amino acids Y114-A436) was used as the immunogen for the Pyruvate dehydrogenase kinase 1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the Pyruvate dehydrogenase kinase 1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the Pyruvate dehydrogenase kinase 1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for PDK1

Target: PDK1 (Pyruvate Dehydrogenase Kinase 1 (PDK1))

Alternative Name: Pyruvate dehydrogenase kinase 1

Background: Pyruvate dehydrogenase lipoamide kinase isozyme 1, mitochondrial is an enzyme that in humans is encoded by the PDK1 gene. Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation. Multiple alternatively spliced transcript variants have been found for this gene.

UniProt: Q15118

Pathways: PI3K-Akt Signaling, RTK Signaling, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Warburg Effect