ERCC5 antibody (AA 115-964)

Catalog No. ABIN7870213

The Rabbit Polyclonal anti-ERCC5 antibody (ABIN7870213) specifically detects ERCC5 in WB, ELISA, IHC, IF, ICC and FACS. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for ERCC5 antibody (AA 115-964) (ABIN7870213)

Target: ERCC5 (DNA Repair Protein Complementing XP-G Cells (ERCC5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ERCC5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-ERCC5 Antibody

Binding Specificity: AA 115-964

Purpose: ERCC5 Antibody / Excision repair cross-complementation group 5

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human XPG/ERCC5 recombinant protein (Position: K115-R964) was used as the immunogen for the ERCC5 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the ERCC5 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the ERCC5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for ERCC5

Target: ERCC5 (DNA Repair Protein Complementing XP-G Cells (ERCC5))

Alternative Name: ERCC5

Background: ERCC5 antibody detects Excision repair cross-complementation group 5 protein, a structure-specific endonuclease essential for nucleotide excision repair (NER). Also known as Xeroderma pigmentosum group G protein (XPG), ERCC5 catalyzes 3' incision at sites of DNA damage, removing bulky lesions such as ultraviolet-induced pyrimidine dimers. The ERCC5 antibody is widely used in DNA repair, cancer, and dermatological research to study genome stability, DNA damage responses, and repair-deficient disorders.

ERCC5 is encoded by the ERCC5 gene located on human chromosome 13q33.1. The protein is approximately 118 kilodaltons in size and belongs to the XPG/RAD2 family of structure-specific nucleases. It contains conserved N- and I-domains forming the catalytic core, as well as C-terminal regions mediating interactions with transcription and repair factors such as TFIIH and PCNA. Through these interactions, ERCC5 coordinates incision with repair synthesis during the NER process.

The ERCC5 antibody typically detects a strong nuclear band near 133 kilodaltons by western blot. Immunofluorescence reveals nuclear localization, often enriched at DNA damage foci following UV or oxidative stress exposure. Functional studies demonstrate that ERCC5 excises damaged DNA downstream of the lesion, allowing DNA polymerases to resynthesize the correct strand. Mutations in ERCC5 cause Xeroderma pigmentosum complementation group G (XP-G) and Cockayne syndrome, conditions characterized by UV hypersensitivity, neurological abnormalities, and premature aging.

Beyond its canonical repair function, ERCC5 also contributes to transcription-coupled repair and RNA polymerase II-mediated gene expression. It participates in DNA replication stress responses and protects replication forks from collapse under genotoxic stress. Dysregulation or reduced expression of ERCC5 has been linked to increased cancer susceptibility, particularly skin, liver, and lung cancers. NSJ Bioreagents offers a validated ERCC5 antibody optimized for western blot, immunohistochemistry, and DNA damage studies, providing researchers with a reliable tool for analyzing DNA repair mechanisms, NER efficiency, and genome protection pathways.

UniProt: P28715

Pathways: DNA Damage Repair