SRRT antibody (AA 125-793)

Catalog No. ABIN7870381

The Rabbit Polyclonal anti-SRRT antibody (ABIN7870381) specifically detects SRRT in WB, ELISA, IHC, IP, IF, FACS and ICC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for SRRT antibody (AA 125-793) (ABIN7870381)

Target: SRRT (Serrate RNA Effector Molecule Homolog (SRRT))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SRRT antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-SRRT Antibody

Binding Specificity: AA 125-793

Purpose: SRRT Antibody / ARS2

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human ARS2/SRRT recombinant protein (Position: H125-Q793) was used as the immunogen for the SRRT antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the SRRT antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the SRRT antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for SRRT

Target: SRRT (Serrate RNA Effector Molecule Homolog (SRRT))

Alternative Name: SRRT

Background: SRRT antibody detects Serrate RNA effector molecule homolog, also known as ARS2, encoded by the SRRT gene on chromosome 7q22.1. SRRT antibody is widely used in studies of RNA metabolism, microRNA processing, and transcriptional regulation. SRRT/ARS2 functions as a nuclear cap-binding complex (CBC)-associated factor, linking transcription termination with RNA processing. It is expressed across many tissues, with elevated levels in proliferating cells where RNA synthesis and processing are highly active.

Structurally, SRRT contains RNA-binding domains and conserved regions that mediate interactions with the cap-binding complex proteins CBP80 and CBP20. These interactions stabilize RNA transcripts and recruit processing machinery. SRRT also associates with the microprocessor complex component DROSHA, supporting microRNA biogenesis. Alternative splicing generates isoforms with distinct regulatory functions.

Functionally, SRRT/ARS2 acts as a multifunctional adaptor at the intersection of transcription and RNA processing. It coordinates transcription termination, 3' end formation, and maturation of non-coding RNAs. SRRT also regulates microRNA production by stabilizing pri-miRNA transcripts and facilitating their cleavage by the DROSHA-DGCR8 complex. Knockdown experiments reduce microRNA levels, impairing gene silencing and altering cell proliferation. Researchers use SRRT antibody to study RNA metabolism, transcription regulation, and microRNA biology.

Clinically, SRRT has been implicated in cancer and developmental disorders. Overexpression of SRRT supports tumor cell proliferation by enhancing RNA processing efficiency. Mutations or deletions of SRRT disrupt RNA maturation and contribute to developmental abnormalities. SRRT has also been linked to antiviral defense, where it regulates RNA stability during infection. NSJ Bioreagents provides SRRT antibody to support research in RNA biology, oncology, and developmental disease.

Experimentally, SRRT antibody is used in western blotting to detect the ~100 kDa protein, in immunofluorescence microscopy to study nuclear localization, and in immunohistochemistry to evaluate tissue-specific expression. Co-immunoprecipitation with SRRT antibody enables identification of RNA processing complexes and binding partners.

UniProt: Q9BXP5

Pathways: Notch Signaling, Stem Cell Maintenance