PLCD1 antibody (AA 14-549)

Catalog No. ABIN7870647

The Rabbit Polyclonal anti-PLCD1 antibody is suitable to detect PLCD1 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA and FACS.

Quick Overview for PLCD1 antibody (AA 14-549) (ABIN7870647)

Target: PLCD1 (phospholipase C, delta 1 (PLCD1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PLCD1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-PLCD1 Antibody

Binding Specificity: AA 14-549

Purpose: PLCD1 Antibody / Phospholipase C delta 1

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human PLCD1 recombinant protein (Position: Q14-R549) was used as the immunogen for the PLCD1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the PLCD1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the PLCD1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for PLCD1

Target: PLCD1 (phospholipase C, delta 1 (PLCD1))

Alternative Name: PLCD1

Background: PLCD1 antibody detects Phospholipase C delta 1, a signaling enzyme that hydrolyzes phosphatidylinositol 4,5-bisphosphate (PIP2) into secondary messengers inositol trisphosphate (IP3) and diacylglycerol (DAG). The UniProt recommended name is Phospholipase C delta 1 (PLCD1). This enzyme participates in calcium signaling and regulates diverse cellular processes such as growth, differentiation, and cytoskeletal reorganization.

Functionally, PLCD1 antibody identifies a 756-amino-acid enzyme containing an N-terminal PH domain for membrane binding, EF-hand motifs for calcium interaction, a catalytic X-Y domain for phosphodiesterase activity, and a C2 domain for phospholipid association. Upon receptor stimulation, PLCD1 catalyzes the cleavage of PIP2, generating IP3 to release intracellular calcium and DAG to activate protein kinase C (PKC).

The PLCD1 gene is located on chromosome 3p22.3 and is expressed in epithelial tissues, brain, and smooth muscle. It functions downstream of receptor tyrosine kinases and G protein-coupled receptors to mediate signal transduction. PLCD1 contributes to actin cytoskeleton remodeling and cell migration through localized phosphoinositide metabolism.

Pathologically, downregulation or mutation of PLCD1 is associated with cancer progression, skin disorders, and developmental defects. PLCD1 acts as a tumor suppressor in certain epithelial cancers by modulating calcium signaling and cell adhesion. Research using PLCD1 antibody supports studies in signal transduction, lipid metabolism, and cell communication.

PLCD1 antibody is validated for western blotting, immunohistochemistry, and immunofluorescence to detect phospholipase enzymes in signaling pathways. NSJ Bioreagents provides PLCD1 antibody reagents optimized for cell signaling, cancer research, and phosphoinositide biology.

Structurally, Phospholipase C delta 1 contains catalytic and regulatory domains that coordinate substrate recognition and calcium-dependent activity. This antibody facilitates investigation of PLCD1's role in intracellular signaling and calcium homeostasis.

UniProt: P51178

Pathways: WNT Signaling, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process