SPHK1 antibody (AA 16-267)

Catalog No. ABIN7871063

This Rabbit Polyclonal antibody specifically detects SPHK1 in WB, ELISA, FACS, IHC (p) and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for SPHK1 antibody (AA 16-267) (ABIN7871063)

Target: SPHK1 (Sphingosine Kinase 1 (SPHK1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPHK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Product Details anti-SPHK1 Antibody

Binding Specificity: AA 16-267

Purpose: SPHK1 Antibody / Sphingosine Kinase 1

Purification: Antigen affinity purified

Immunogen: Recombinant human protein (amino acids R16-H267) was used as the immunogen for the SPHK1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the SPHK1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the SPHK1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for SPHK1

Target: SPHK1 (Sphingosine Kinase 1 (SPHK1))

Alternative Name: SPHK1

Background: SPHK1 (Sphingosine Kinase 1), is an enzyme that in humans is encoded by the SPHK1 gene. Melendez et al.(2000) mapped the SPHK1 gene to chromosome 17q25.2 based on sequence identity with ESTs mapped to this region. Kohama et al.(1998) demonstrated that recombinant mouse Sphk1 can specifically phosphorylate D-erythro-sphingosine and that D, L-threo-dihydrosphingosine and N, N-dimethylsphingosine can act as competitive inhibitors of recombinant Sphk1. Pitson et al.(2000) found that recombinant SPHK1 and endogenous SPHK1 purified from placenta had identical enzymatic characteristics, suggesting posttranslational modification does not effect functional properties.

UniProt: Q9NYA1

Pathways: VEGF Signaling