TOP1MT antibody (AA 18-601)

Catalog No. ABIN7871431

The Rabbit Polyclonal anti-TOP1MT antibody (ABIN7871431) specifically detects TOP1MT in IHC, WB, FACS and ELISA. The antibody is reactive with Human and Mouse samples.

Quick Overview for TOP1MT antibody (AA 18-601) (ABIN7871431)

Target: TOP1MT (Topoisomerase (DNA) I, Mitochondrial (TOP1MT))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TOP1MT antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Flow Cytometry (FACS), ELISA

Product Details anti-TOP1MT Antibody

Binding Specificity: AA 18-601

Purpose: TOP1MT Antibody / DNA topoisomerase 1 mitochondrial

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human TOP1MT recombinant protein (Position: E18-F601) was used as the immunogen for the TOP1MT antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the TOP1MT antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the TOP1MT antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for TOP1MT

Target: TOP1MT (Topoisomerase (DNA) I, Mitochondrial (TOP1MT))

Alternative Name: TOP1MT

Background: TOP1MT antibody detects DNA topoisomerase 1 mitochondrial, encoded by the TOP1MT gene. DNA topoisomerase 1 mitochondrial is an enzyme located in the mitochondrial matrix that relaxes supercoiled mitochondrial DNA, enabling replication, transcription, and repair. TOP1MT antibody provides researchers with a specific reagent for studying mitochondrial DNA metabolism, genome maintenance, and disease.

DNA topoisomerase 1 mitochondrial functions by introducing transient single-strand breaks in DNA, relieving torsional stress during replication and transcription. Research using TOP1MT antibody has shown that this enzyme maintains mitochondrial genome integrity by resolving supercoils generated during transcription of mitochondrial genes. Its activity is essential for proper mitochondrial gene expression and energy production.

Studies with TOP1MT antibody have revealed that loss of TOP1MT disrupts mitochondrial transcription and translation, impairing oxidative phosphorylation. This leads to reduced ATP generation, altered mitochondrial morphology, and increased oxidative stress. These phenotypes underscore its central role in mitochondrial biology.

Dysregulation of DNA topoisomerase 1 mitochondrial has been associated with human disease. Research using TOP1MT antibody has shown that variants can contribute to mitochondrial dysfunction, neuromuscular disorders, and cardiomyopathies. Furthermore, reduced TOP1MT activity exacerbates mitochondrial genome instability, linking it to degenerative and metabolic diseases.

Beyond mitochondrial maintenance, TOP1MT has been implicated in cellular stress responses and cancer. Research using TOP1MT antibody has shown that altered expression influences sensitivity to chemotherapy agents targeting topoisomerases, suggesting potential relevance in therapeutic response.

TOP1MT antibody is widely applied in immunohistochemistry, western blotting, and mitochondrial fractionation studies. Immunohistochemistry demonstrates mitochondrial localization, western blotting quantifies expression across tissues, and fractionation confirms enrichment in mitochondria. These applications make TOP1MT antibody indispensable in mitochondrial research.

By supplying validated TOP1MT antibody reagents, NSJ Bioreagents supports studies into mitochondrial genome regulation, oxidative phosphorylation, and disease. Detection of DNA topoisomerase 1 mitochondrial provides researchers with insight into how mitochondrial enzymes preserve genome stability and energy metabolism.

UniProt: Q969P6