Melanoma gp100 antibody (AA 182-5565)

Catalog No. ABIN7871468

This Rabbit Polyclonal antibody specifically detects Melanoma gp100 in IHC, WB, FACS, IF and ELISA. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for Melanoma gp100 antibody (AA 182-5565) (ABIN7871468)

Target: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Melanoma gp100 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), ELISA

Product Details anti-Melanoma gp100 Antibody

Binding Specificity: AA 182-5565

Purpose: PMEL Antibody / Premelanosome protein / SILV

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human SILV/PMEL recombinant protein (Position: H182-Q5565) was used as the immunogen for the PMEL antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the PMEL antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the PMEL antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for Melanoma gp100

Target: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Alternative Name: PMEL

Background: PMEL antibody detects Premelanosome protein, a structural component of melanosomes essential for melanin synthesis and pigment formation. The UniProt recommended name is Premelanosome protein (PMEL). Also known as SILV, gp100, or ME20, this glycoprotein forms amyloid fibrils that serve as scaffolds for melanin polymerization within developing melanosomes.

Functionally, PMEL antibody identifies a 661-amino-acid type I transmembrane glycoprotein synthesized in melanocytes. PMEL is cleaved by proprotein convertases into M alpha and M beta fragments, which assemble into amyloid fibrils in stage II melanosomes. These fibrils provide a matrix for eumelanin deposition, enabling proper pigment granule maturation.

The PMEL gene is located on chromosome 12p13.31 and is expressed exclusively in pigment-producing cells, including melanocytes and retinal pigment epithelium. PMEL expression is regulated by the transcription factor MITF and is upregulated during melanocyte differentiation. It plays a central role in pigment biogenesis and melanosome architecture.

Pathologically, PMEL is a target antigen in melanoma immunotherapy and an established marker for melanocytic tumors. Mutations in PMEL can cause pigment dilution phenotypes in animals due to altered fibril formation. Research using PMEL antibody supports studies in pigmentation biology, melanoma diagnostics, and organelle structure.

PMEL antibody is validated for immunohistochemistry, western blotting, and immunofluorescence to detect melanosomal structural proteins. NSJ Bioreagents provides PMEL antibody reagents optimized for pigment biology, cancer research, and cell differentiation studies.

Structurally, Premelanosome protein contains an N-terminal signal peptide, a polycystic kidney disease-like (PKD) domain, and a transmembrane domain. Its cleaved fragments aggregate to form functional amyloid fibrils. This antibody supports detailed examination of PMEL's role in melanosome assembly and pigmentation control.

UniProt: P40967