SMPD3 antibody (AA 184-553)

Catalog No. ABIN7871479

The Rabbit Polyclonal anti-SMPD3 antibody (ABIN7871479) specifically detects SMPD3 in WB and ELISA. The antibody is reactive with Human samples.

Quick Overview for SMPD3 antibody (AA 184-553) (ABIN7871479)

Target: SMPD3 (Sphingomyelin Phosphodiesterase 3 (SMPD3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMPD3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-SMPD3 Antibody

Binding Specificity: AA 184-553

Purpose: SMPD3 Antibody / Sphingomyelin phosphodiesterase 3 / nSMase2

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human SMPD3 recombinant protein (Position: F184-D553) was used as the immunogen for the SMPD3 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the SMPD3 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the SMPD3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for SMPD3

Target: SMPD3 (Sphingomyelin Phosphodiesterase 3 (SMPD3))

Alternative Name: SMPD3

Background: SMPD3 antibody detects Sphingomyelin phosphodiesterase 3, a membrane-associated enzyme that hydrolyzes sphingomyelin to generate ceramide and phosphocholine. The UniProt recommended name is Sphingomyelin phosphodiesterase 3 (SMPD3). This enzyme, also known as neutral sphingomyelinase 2 (nSMase2), plays a key role in lipid metabolism, cell signaling, and exosome biogenesis.

Functionally, SMPD3 antibody identifies a 655-amino-acid enzyme localized to the plasma membrane and Golgi apparatus. SMPD3 catalyzes the production of ceramide, a lipid second messenger involved in apoptosis, differentiation, and inflammation. It regulates membrane curvature and vesicle budding, particularly in exosome secretion and cell stress responses.

The SMPD3 gene is located on chromosome 16p13.12 and is expressed in brain, liver, and skeletal tissue. SMPD3 activity influences cell growth, neuronal communication, and lipid raft organization. By controlling ceramide levels, SMPD3 links sphingolipid metabolism to cell fate decisions and inflammatory signaling.

Pathologically, dysregulation of SMPD3 contributes to metabolic, neurodegenerative, and cardiovascular diseases. Deficiency results in skeletal abnormalities (spondyloepimetaphyseal dysplasia) and altered lipid homeostasis. Overactivation can enhance ceramide-induced apoptosis or inflammatory cascades. Research using SMPD3 antibody supports studies in lipid signaling, neurobiology, and exosome biology.

SMPD3 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect sphingomyelinases and lipid signaling enzymes. NSJ Bioreagents provides SMPD3 antibody reagents optimized for cell signaling, metabolism, and membrane biology research.

Structurally, Sphingomyelin phosphodiesterase 3 contains an N-terminal hydrophobic domain for membrane association and a C-terminal catalytic domain requiring Mg2+ for activity. This antibody enables analysis of SMPD3i 1/2s role in ceramide-mediated signal transduction and cellular stress responses.

UniProt: Q9NY59

Pathways: Hormone Transport