NPM1 antibody (AA 185-287)

Catalog No. ABIN7871495

This Mouse Monoclonal antibody specifically detects NPM1 in WB and IHC (p). It exhibits reactivity toward Human.

Quick Overview for NPM1 antibody (AA 185-287) (ABIN7871495)

Target: NPM1 (Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NPM1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: NLPP1-1

Product Details anti-NPM1 Antibody

Binding Specificity: AA 185-287

Purpose: NPM Antibody / Nucleophosmin

Purification: Protein G affinity chromatography

Immunogen: A portion of amino acids 185-287 from the human protein was used as the immunogen for the NPM antibody.

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal dilution of the NPM antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the NPM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for NPM1

Target: NPM1 (Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1))

Alternative Name: NPM

Background: The protein encoded by the NPM1 gene is involved in several cellular processes, including centrosome duplication, protein chaperoning, and cell proliferation. The encoded phosphoprotein shuttles between the nucleolus, nucleus, and cytoplasm, chaperoning ribosomal proteins and core histones from the nucleus to the cytoplasm. This protein is also known to sequester the tumor suppressor ARF in the nucleolus, protecting it from degradation until it is needed. Mutations in this gene are associated with acute myeloid leukemia. [RefSeq]

UniProt: P06748

Pathways: Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly