MPG antibody (AA 20-298)

Catalog No. ABIN7871858

This Rabbit Polyclonal antibody specifically detects MPG in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for MPG antibody (AA 20-298) (ABIN7871858)

Target: MPG (N-Methylpurine-DNA Glycosylase (MPG))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MPG antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-MPG Antibody

Binding Specificity: AA 20-298

Purpose: DNA-3-methyladenine glycosylase Antibody / MPG / AAG

Purification: Antigen affinity purified

Immunogen: Recombinant human protein (amino acids Q20-A298) was used as the immunogen for the DNA-3-methyladenine glycosylase antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the DNA-3-methyladenine glycosylase antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the DNA-3-methyladenine glycosylase antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for MPG

Target: MPG (N-Methylpurine-DNA Glycosylase (MPG))

Alternative Name: DNA-3-methyladenine glycosylase

Background: MPG (N-methylpurine-DNA glycosylase) also known as MDG, 3-METHYLADENINE DNA GLYCOSYLASE, 3MeAde DNA GLYCOSYLASE, AAG and APNG is a protein expressed in all cell lines and tissues examined, but was found at particularly high levels in a colon adenocarcinoma cell line(HT29). The completely characterized human MPG gene was found to span 7 to 8 kb of genomic DNA and to be localized 75 kb upstream of the embryonic zeta-globin gene. To assess the contribution of Apng to the repair of several mutagenic lesions in vivo,Hang et al.(1997)biochemically analyzed cell-free extracts of tissues from mice with a targeted deletion of the Apng gene. Following treatment with DNA-methylating agents, increased persistence of 7-methylguanine (meG) was found in liver sections of APNG knockout mice in comparison with wildtype mice, demonstrating an in vivo phenotype for the APNG-null animals.

UniProt: P29372

Pathways: DNA Damage Repair