LAT antibody (AA 28-235)

Catalog No. ABIN7873245

The Rabbit Polyclonal anti-LAT antibody is suitable to detect LAT in samples from Human. It has been validated for WB, ELISA and FACS.

Quick Overview for LAT antibody (AA 28-235) (ABIN7873245)

Target: LAT (Linker For Activation of T Cells (LAT))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LAT antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-LAT Antibody

Binding Specificity: AA 28-235

Purpose: LAT Antibody / Linker for activation of T-cells family member 1

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human LAT recombinant protein (Position: H28-E235) was used as the immunogen for the LAT antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the LAT antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the LAT antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for LAT

Target: LAT (Linker For Activation of T Cells (LAT))

Alternative Name: LAT

Background: LAT antibody detects Linker for activation of T-cells family member 1, encoded by the LAT gene. Linker for activation of T-cells family member 1 is a transmembrane adaptor protein expressed in T cells and natural killer cells, where it serves as a central scaffold for antigen receptor signaling. LAT antibody provides researchers with a critical reagent for studying T cell receptor signaling, immune responses, and related diseases.

Linker for activation of T-cells family member 1 localizes to lipid rafts in the plasma membrane. Research using LAT antibody has shown that upon T cell receptor stimulation, LAT becomes tyrosine-phosphorylated by ZAP70. These phosphorylated residues recruit multiple signaling proteins, including GRB2, PLCG1, and GADS, forming a large signaling complex. This assembly drives calcium signaling, MAPK activation, and transcription of immune effector genes.

Studies with LAT antibody have demonstrated that LAT is indispensable for T cell development and activation. Knockout models lacking LAT fail to develop mature T cells, and mutations in LAT impair signaling pathways required for adaptive immunity. These findings emphasize its role as a master regulator of immune receptor signaling.

Dysregulation of LAT contributes to disease. Research using LAT antibody has revealed that mutations in the LAT gene cause severe combined immunodeficiency, characterized by absent or impaired T cell function. Aberrant LAT signaling has also been implicated in autoimmune disease, where inappropriate activation leads to tissue damage. In oncology, altered LAT expression influences T cell activity within the tumor microenvironment, affecting anti-tumor immunity.

LAT antibody is widely used in immunoprecipitation, immunoblotting, and flow cytometry. Immunoprecipitation identifies signaling complexes assembled at LAT, immunoblotting detects phosphorylated and total protein levels, and flow cytometry quantifies expression in T cell populations. These applications make LAT antibody indispensable in immunology research.

By supplying validated LAT antibody reagents, NSJ Bioreagents supports studies into T cell receptor signaling, immune development, and pathology. Detection of Linker for activation of T-cells family member 1 provides researchers with insight into how scaffold proteins regulate receptor signaling networks.

UniProt: O43561

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway