Leptin Receptor antibody (AA 335-425)

Catalog No. ABIN7874044

The Mouse Monoclonal anti-Leptin Receptor antibody (Clone LEPR-4545) (ABIN7874044) specifically detects Leptin Receptor in IHC (p). The antibody is reactive with Human samples.

Quick Overview for Leptin Receptor antibody (AA 335-425) (ABIN7874044)

Target: Leptin Receptor (LEPR)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Leptin Receptor antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: LEPR-4545

Product Details anti-Leptin Receptor Antibody

Binding Specificity: AA 335-425

Purpose: Ob-R Antibody / Leptin Receptor / LEPR

Purification: Protein A/G affinity

Immunogen: A recombinant partial protein sequence (within amino acids 335-425) from the human protein was used as the immunogen for the Ob-R antibody.

Isotype: IgG2b, kappa

Application Details

Application Notes: Optimal dilution of the Ob-R antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the Ob-R antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for Leptin Receptor

Target: Leptin Receptor (LEPR)

Alternative Name: Ob-R

Background: Although there is substantial evidence that body weight is physiologically regulated, the molecular basis of obesity is unknown. Five single-gene mutations in mice that result in an obese phenotype have been identified. The first such recessive obesity mutation, the obese mutation (Ob), was identified in 1950. Mutation of Ob results in profound obesity and type II diabetes as part of a syndrome that resembles morbid obesity in humans. It has been postulated that the Ob gene product may function as a component of a signaling pathway in adipose tissue that functions to regulate body fat depot size. The cloning and sequence analysis of the mouse Ob gene and its human homolog has recently been described. Ob encodes an adipose tissue-specific mRNA with a highly conserved 167 amino acid open reading frame. The predicted amino acid sequence is 84 % identical between human and mouse and has the features of a secreted protein. A nonsense mutation in codon 105 has been found in the original congenic C57BL/6J Ob/Ob mouse strain. The Ob gene encodes the protein leptin. The leptin receptor, designated Ob-R, has been shown to be a single membrane-spanning receptor that most resembles the gp130 signal transducing component of the IL-6, G-CSF and LIF receptor. Ob-R mRNA is expressed in the choroid plexus and hypothalamus.

UniProt: P48357

Pathways: JAK-STAT Signaling, AMPK Signaling, Feeding Behaviour